[KO Validated] Anti-IGF2BP1/IMP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NZI8

Core Product Specifications and Parameters

Biological Background: IGF2BP1 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), also known as IMP1, ZBP1, CRD-BP, and VICKZ1, is a member of the VICKZ family of RNA-binding proteins.
• It functions as an RNA-binding protein that recruits target transcripts to cytoplasmic mRNPs, modulating their transport, stability, and translation. IGF2BP1 preferentially binds to m6A-modified mRNAs, enhancing their stability. Related references: PMID:29476152, PMID:32245947
• IGF2BP1 binds to the coding region instability determinant (CRD) of MYC mRNA, preventing endonucleolytic cleavage and microRNA-mediated degradation; this interaction is promoted by m6A modification of the CRD. Related references: PMID:29476152
• It plays a critical role in the spatiotemporal regulation of β-actin (ACTB) mRNA translation, which is essential for cell polarity, migration, neurite outgrowth, and axonal regeneration.
• Subcellularly, IGF2BP1 localizes to the nucleus, cytoplasm, stress granules, P-bodies, and various cell protrusions (lamellipodia, filopodia, dendrites, growth cones, axons), consistent with its roles in mRNA transport and local translation.
• The protein is predominantly expressed in embryonic tissues and is upregulated in many cancers, including lung, testicular, and colorectal cancers, where it contributes to tumor cell migration and invasion.
• IGF2BP1 is phosphorylated, which regulates its release of bound mRNAs. It is also involved in alternative splicing, mRNA transport, translation regulation, and synaptic function, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human IMP1 (NP_006537.3). Consider verifying isoform specificity in your sample system, as multiple isoforms exist.
• This antibody is validated for Western blot (WB) and ELISA. For WB, expect a band around 63 kDa, but migration may shift due to phosphorylation or isoform variation.
• Reported cross-reactivity with mouse and rat enables comparative studies, but validation in the specific species and tissue is recommended.
• As IGF2BP1 associates with stress granules, consider sample preparation conditions (e.g., inclusion of RNase inhibitors, reducing agents) to optimize detection.
• For ELISA applications, titrate the antibody to determine optimal working concentrations; pairing with other detection reagents may require empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement platform for IVD raw materials, including validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-IGF2BP1 antibody supports research in RNA biology, cancer biomarker discovery, and neurobiology. We bridge the gap from concept to clinic, providing reliable sourcing solutions for diagnostic developers and research laboratories alike.