[KO Validated] Anti-Hsp90β Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08238

Core Product Specifications and Parameters

Biological Background: Hsp90β Function and Localization

• HSP90β (HSP90AB1) is a molecular chaperone that promotes the maturation, structural maintenance, and proper regulation of client proteins involved in cell cycle control and signal transduction. It undergoes an ATPase-dependent functional cycle, interacting with various co-chaperones to modulate substrate recognition and chaperone activity. Related references: PMID:16478993 PMID:19696785 PMID:26991466 PMID:27295069

• Apart from chaperone activity, HSP90β regulates transcription by altering transcription factor steady-state levels, modulating epigenetic modifiers like histone deacetylases, and evicting histones from promoter regions to turn on gene expression. Related references: PMID:25973397

• HSP90β antagonizes STUB1-mediated inhibition of TGF-beta signaling by preventing STUB1-dependent SMAD3 ubiquitination and degradation. Related references: PMID:24613385

• It promotes cell differentiation by chaperoning BIRC2, protecting it from auto-ubiquitination and proteasomal degradation. Related references: PMID:18239673

• Under heat shock, HSP90β chaperones both JAK2 and PRKCE, leading to phosphorylation and activation of STAT1, and in turn activates its own transcription. Related references: PMID:20353823

• Involved in the translocation of leaderless cargos (e.g., interleukin 1/IL-1) into the ER-Golgi intermediate compartment (ERGIC) via the cargo receptor TMED10. Related references: PMID:32272059

• Localizes predominantly in the cytoplasm but can also be found in melanosomes, nucleus, secreted, and associated with cell membranes and dynein axonemal particles. Subject to post-translational modifications including acetylation, phosphorylation, methylation, S-nitrosylation, and ubiquitination.

• (Microbial infection) Binds to N.meningitidis NadA, stimulating monocytes, and seems to interfere with NadA-mediated invasion of human cells. Related references: PMID:21949862

Experimental Guidance and Technical Tips

• For Western blot, the expected band is at ~83 kDa. Use standard SDS-PAGE under reducing conditions, and consider optimizing blocking and antibody dilution for your sample type. Validate specificity using Hsp90β knockout cell lines if available.
• For immunofluorescence (IF/ICC), test various fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) protocols, as HSP90β can be cytoplasmic, nuclear, or membrane-associated depending on cell context.
• The immunogen covers amino acids 1-300 (N-terminal region) of human Hsp90β; note that this region includes the ATP-binding domain, which is highly conserved. Cross-reactivity with mouse, rat, and monkey is expected based on supplier data, but it is advisable to verify for your specific application.
• For ELISA, consider optimizing coating antigen and detection system; the antibody is a polyclonal, so batch-to-batch consistency should be monitored.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing validated antibodies, including polyclonal and monoclonal options against key targets such as HSP90β, a central chaperone in cancer and stress responses. Our sourcing bridge connects diagnostic manufacturers with bulk ancillary reagents and rare-target antibody access, streamlining your development from concept to clinic. Let us support your next project with reliable supply and technical expertise.