[KO Validated] Anti-Hsp90α Monoclonal Antibody for WB, ELISA - P07900

Core Product Specifications and Parameters

Biological Background: Hsp90α Function and Localization

• Hsp90α is an ATP-dependent molecular chaperone that facilitates the maturation and functional regulation of client proteins involved in cell cycle control and signal transduction. Related references: PMID:11274138, PMID:12526792, PMID:15577939
• It operates through a dynamic cycle of ATP binding and hydrolysis, regulating client protein conformation via interaction with co-chaperones. Related references: PMID:29127155, PMID:26991466, PMID:27295069
• Hsp90α mediates the delivery of preproteins to the mitochondrial import receptor TOMM70, playing a critical role in mitochondrial protein import. Related references: PMID:12526792
• The protein modulates transcription at multiple levels: altering transcription factor stability, influencing epigenetic modifiers such as HDACs and DNMTs, and facilitating histone eviction from gene promoters. Related references: PMID:25973397
• Hsp90α binds bacterial lipopolysaccharide (LPS) and triggers inflammatory responses including TNF secretion from monocytes. Related references: PMID:11276205
• It associates with TOMM70, IRF3 and TBK1 at the mitochondrial outer membrane to promote innate antiviral signaling. Related references: PMID:20628368, PMID:25609812
• Subcellularly, Hsp90α is found in the nucleus, cytoplasm, melanosome, cell membrane, and mitochondrion, reflecting its diverse functional roles.
• Additional keywords: The protein is subject to acetylation, phosphorylation, S-nitrosylation, and ubiquitin conjugation, which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen (aa 433-732) spans a conserved region, likely contributing to cross-reactivity with mouse and rat Hsp90α. For species-specific detection, verify via sequence alignment.
• Western blot under reducing conditions may reveal bands at ~84 and ~98 kDa; the upper band may reflect post-translational modifications. Use appropriate protein ladders and confirm identity with KO-validated lysates.
• For ELISA, consider using a recombinant Hsp90α standard and optimize coating/detection conditions to minimize LPS-related background.
• KO validation ensures specificity; always include wild-type and knockout cell lysates as critical controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with a comprehensive pipeline of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. For Hsp90α-related research and assay development, we offer bulk quantities and specialized sourcing support to streamline your workflow from concept to clinic.