[KO Validated] Anti-HPRT1 Rabbit Polyclonal Antibody for WB, ELISA - P00492

Core Product Specifications and Parameters

Biological Background: HPRT1 Function and Localization

• HPRT1 encodes hypoxanthine-guanine phosphoribosyltransferase (HGPRT), a central enzyme in the purine salvage pathway.
• The enzyme catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP) and guanine to guanosine monophosphate (GMP) using 5-phosphoribosyl-1-pyrophosphate (PRPP) as the phosphoribosyl donor.
• Primarily localizes to the cytoplasm, where it mediates the recycling of purine bases from degraded nucleic acids.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability.
• Metal binding (magnesium) and nucleotide binding are critical for catalytic function, as indicated by UniProt keywords.
• Mutations in HPRT1 are associated with disease phenotypes, including gout, underscoring its physiological importance in purine metabolism.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–218 of full-length human HPRT1, suggesting broad epitope recognition for detection.
• For Western blot (WB), a specific band at approximately 25 kDa is expected; validate using relevant positive and negative control lysates.
• Knockout validation (KO) of the antibody enhances confidence in target specificity; consider including KO cell lines or tissues in experimental designs.
• ELISA applications can be optimized by pairing with appropriate secondary antibodies and using standard curves derived from recombinant protein.
• Cross-reactivity with mouse and rat orthologs allows multi-species studies, but verify batch-to-batch consistency and perform species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with verified raw materials for IVD development, including antibodies targeting key metabolic enzymes like HPRT1. We offer comprehensive supply solutions encompassing validated polyclonal and monoclonal antibodies, recombinant antigens, and ancillary reagents to accelerate purine salvage pathway research and assay design. For rare or customized targets, our global sourcing network provides reliable access to specialized raw materials, ensuring seamless project progression from concept to clinical application.