[KO Validated] Anti-HNRNPA2B1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P22626

Core Product Specifications and Parameters

Biological Background: HNRNPA2B1 Function and Localization

• HNRNPA2B1 is a heterogeneous nuclear ribonucleoprotein that packages nascent pre-mRNAs into hnRNP particles, condensing and stabilizing transcripts. Related references: PMID:19099192
• It acts as a nuclear reader of N6-methyladenosine (m6A) marks on pri-miRNAs and pre-mRNAs, regulating miRNA processing and splicing efficiency. Related references: PMID:26321680
• Binds specifically to the A2RE sequence motif, mediating mRNA transport to the cytoplasm in oligodendrocytes and neurons, and facilitates HIV-1 genomic RNA transport during infection. Related references: PMID:10567417
• Regulates alternative splicing of pyruvate kinase PKM by repressing exon 9 inclusion, promoting the PKM2 isoform. Related references: PMID:20010808
• Senses viral DNA in the nucleus, homodimerizes, and after demethylation by JMJD6, translocates to the cytoplasm to activate the TBK1-IRF3 innate immune pathway, inducing interferon production. Related references: PMID:31320558
• Involved in sorting miRNAs into exosomes through sumoylation, potentially recognizing m6A marks. Related references: PMID:24356509
• Localizes to the nucleus, nucleoplasm, cytoplasm, cytoplasmic granules, and is secreted in extracellular exosomes, reflecting its dynamic roles in RNA metabolism and intercellular communication.
• Post-translationally modified by acetylation, methylation, phosphorylation, sumoylation, and isopeptide bond formation, regulating its function and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-180 of human HNRNPA2B1 (includes the N-terminal RNA recognition motifs). For Western blot, the antibody may detect multiple isoforms or post-translationally modified forms; consider using appropriate controls.
• For IHC-P and IF/ICC, optimize antigen retrieval and permeabilization conditions, as endogenous HNRNPA2B1 is predominantly nuclear but can shuttle to the cytoplasm upon certain stimuli.
• When performing ELISA, ensure coating antigen matches the immunogen region; pre-adsorption may be required for multi-species cross-reactivity.
• Validate antibody specificity with KO validation data if available; otherwise, consider using siRNA knockdown in relevant cell lines (e.g., HeLa, MCF7).
• Note: HNRNPA2B1 has a closely related paralog HNRNPA1; check for potential cross-reactivity in applications requiring high isoform specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This HNRNPA2B1 polyclonal antibody supports research in RNA biology, splicing regulation, and viral-host interactions, aligning with CamelBio's commitment to supplying validated antibody pairs, optimized mono/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for your diagnostic and research workflows.