[KO Validated] Anti-hnRNP E1/PCBP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15365

Core Product Specifications and Parameters

Biological Background: hnRNP E1/PCBP1 Function and Localization

• Poly(rC)-binding protein 1 (PCBP1), also known as hnRNP E1 or Alpha-CP1, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.
• PCBP1 functions as a single-stranded nucleic acid binding protein with high affinity for oligo dC sequences, regulating mRNA stability and translation. Related references: PMID:15731341 PMID:7556077 PMID:7607214
• Together with PCBP2, PCBP1 is essential for erythropoiesis, likely through the regulation of mRNA splicing events during red blood cell development.
• In the context of poliovirus infection, PCBP1 collaborates with the viral protein 3CD to initiate viral RNA replication.
• PCBP1 localizes to both the nucleus and cytoplasm, reflecting its roles in RNA processing and transport.
• Tissue expression is prominent in skeletal muscle, thymus, and peripheral blood leukocytes, with lower levels in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal, and thyroid glands.
• Post-translational modifications of PCBP1 include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation (Ubl), which may modulate its activity and interactions.
• The protein has a molecular weight of approximately 37 kDa and contains conserved domains responsible for nucleic acid recognition and binding.

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide within the central region (aa 100-200) of human PCBP1; consider this when designing experiments that require N- or C-terminal epitope recognition.
• For Western blot, endogenous PCBP1 is detected at ~37 kDa; include appropriate positive controls (e.g., HeLa, HEK293, or Jurkat lysates) and validate with knockout or knockdown if needed.
• In IHC-P, optimize antigen retrieval conditions and validate staining specificity using known positive (e.g., skeletal muscle, thymus) and negative control tissues.
• For ELISA, determine optimal coating concentration and antibody dilution using recombinant PCBP1 protein or peptide standard.

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CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-PCBP1, and bulk ancillary reagents for immunoassay development. For targets involved in RNA biology and infectious disease, we offer one-stop sourcing solutions from concept to clinic.