[KO Validated] Anti-Histone H2A.Z Polyclonal Antibody for WB, ELISA - P0C0S5

Core Product Specifications and Parameters

Biological Background: Histone H2A.Z Function and Localization

• Histone H2A.Z is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes, contributing to chromatin structure and dynamics.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosome stability by modulating DNA accessibility within chromatin.
• The protein may be involved in the formation of constitutive heterochromatin and is potentially required for accurate chromosome segregation during cell division.
• Histone H2A.Z function is regulated by a complex set of post-translational modifications, including acetylation, methylation, and ubiquitination, collectively known as the histone code.
• It localizes to the nucleus and is an integral component of the nucleosome core, directly binding to DNA.
• The protein belongs to the histone H2A gene family and is encoded by the H2AZ1 (H2AFZ) gene in humans.
• Keywords associated with P0C0S5 include 3D-structure, acetylation, chromosome, DNA-binding, isopeptide bond, methylation, nucleosome core, nucleus, proteomics identification, and Ubl conjugation.
• Histone H2A.Z has a molecular weight of approximately 13.6 kDa (13553 Da calculated) and is highly conserved across eukaryotes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-128 of human H2AFZ, which encompasses nearly the full-length protein. This broad epitope coverage is suitable for detecting the endogenous target in various applications.
• For Western blotting, a band around 13 kDa is expected. Given the polyclonal nature of the antibody, consider titrating the antibody concentration (e.g., 1:500–1:2000) to optimize signal-to-noise ratio.
• The knockout (KO) validation status indicates that the antibody specificity has been confirmed using KO cell lines; therefore, incorporation of a KO control is recommended for highest confidence in WB assays.
• Cross-reactivity is reported for human, mouse, and rat. Verify performance in your specific sample type and species, as sequence homology may result in comparable detection.
• For ELISA applications, pair with an appropriate secondary antibody and validate coating antigen conditions empirically. This antibody can be used as a capture or detection reagent in sandwich ELISA format.
• Due to the highly conserved nature of histones, consider testing with negative controls (e.g., peptide blocking) to rule out nonspecific binding if unexpected bands appear.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier that connects diagnostic manufacturers, research laboratories, and development teams with high-quality antibodies, antigen pairs, and ancillary reagents. With this KO-validated Histone H2A.Z polyclonal antibody, we support epigenetic and chromatin research, as well as the development of targeted diagnostic tools. Beyond individual antibodies, we offer bulk sourcing of validated monoclonal and polyclonal antibodies, optimized recombinant proteins, and rare target raw materials, ensuring a seamless transition from concept to clinic.