KO Validated Anti-Histone H1.0 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P07305

Core Product Specifications and Parameters

Biological Background: Histone H1.0 Function and Localization

• Histone H1.0 is a linker histone that binds to DNA between nucleosomes, contributing to the formation of chromatin fiber and higher-order chromatin structures. Related references: PMID:33238161

• It facilitates nucleosome chain condensation and promotes the deposition of the H3K27me3 repressive mark via the PRC2/EED-EZH2 complex. Related references: PMID:33238161

• Expression of H1.0 is enriched in terminally differentiated cells or those with low proliferation rates, distinct from replication-dependent H1 variants. Related references: PMID:7374750

• The protein localizes primarily to the nucleus, including nucleoli, and associates with chromosomes during cell division.

• It undergoes multiple post-translational modifications such as acetylation, phosphorylation, ADP-ribosylation, and citrullination, which may regulate its function and chromatin dynamics.

• Alternative splicing of the H1-0 gene generates different isoforms, adding to the functional diversity of histone H1 variants.

• H1.0 possesses strong DNA-binding activity, essential for stabilizing compacted chromatin states.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–105) of human H1F0; confirm that the epitope is accessible in your specific sample preparation and assay conditions.
• For Western blotting, a band near 21 kDa is expected; consider using nuclear-enriched lysates from differentiated cells or tissues as positive controls.
• In IHC-P, validate staining patterns against known H1.0 expression profiles (e.g., low-proliferating, differentiated cells) and include appropriate nuclear counterstains.
• ELISA performance may vary; dilute and titrate according to your standard laboratory protocols and perform side-by-side comparisons with known controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing to diagnostic developers and research institutions worldwide. The Histone H1.0 antibody fits into epigenetics and chromatin biology research applications, supporting assay development in areas such as cancer differentiation markers and cell cycle studies. Leverage our one-stop platform to streamline your supply chain from discovery to clinical use—backed by technical consulting and flexible procurement solutions.