[KO Validated] Anti-HIRA Polyclonal Antibody for WB, IHC-P, ELISA - P54198

Core Product Specifications and Parameters

Biological Background: HIRA Function and Localization

• Protein HIRA, also known as TUP1-like enhancer of split protein 1, is a member of the WD repeat protein family and plays a critical role in chromatin regulation and transcription.
• Cooperates with ASF1A to promote replication-independent chromatin assembly, essential for maintaining chromatin structure outside of S-phase.
• Required for periodic repression of histone gene transcription during the cell cycle, linking chromatin assembly to cell cycle progression.
• Essential for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit, implicating HIRA in cellular aging and tumor suppression.
• Localizes to the nucleus and specifically to PML nuclear bodies, suggesting a role in nuclear organization and protein modification.
• Expressed ubiquitously with high levels in kidney, pancreas, and skeletal muscle, and lower levels in brain, heart, liver, lung, and placenta.
• Post-translationally modified by phosphorylation and ubiquitin-like conjugation (Ubl), which may regulate its activity and interactions.
• Subject to alternative splicing, generating multiple isoforms that could have distinct functions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 250–550 of human HIRA, targeting an internal region; this design minimizes interference from post-translational modifications at the termini.
• This product is knockout (KO) validated, indicating that the antibody signal is specifically abolished in HIRA-deficient samples; it is advisable to include appropriate KO controls in your experiments.
• For Western blot (WB), the predicted molecular weight is approximately 112 kDa; ensure adequate protein separation using a low percentage SDS-PAGE gel (e.g., 8%) and validate with a molecular weight marker.
• In immunohistochemistry (IHC-P), antigen retrieval protocols and antibody dilution should be optimized for your specific tissue type, considering the high expression in kidney, pancreas, and skeletal muscle.
• For ELISA, the antibody can be used as a capture or detection reagent; consider pairing with a matched antibody pair if quantitative detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a comprehensive sourcing solution for IVD raw materials, including validated primary antibodies like this anti-HIRA polyclonal antibody. Our portfolio covers chromatin and cell cycle research targets, supported by bulk reagent supply and technical consulting to accelerate your assay development. From rare target sourcing to custom antibody services, CamelBio streamlines your path from concept to clinic.