KO Validated Anti-Hexokinase II Monoclonal Antibody for WB, ELISA - P52789

Core Product Specifications and Parameters

Biological Background: Hexokinase II Function and Localization

• Hexokinase-2 (HK II), encoded by the HK2 gene, is a 102 kDa protein with two hexokinase domains, acting as a key regulatory enzyme in glucose metabolism.
• Catalyzes the phosphorylation of D-glucose to glucose 6-phosphate, the first committed step of glycolysis. Related references: PMID:23185017, PMID:26985301, PMID:29298880
• Also phosphorylates other hexoses such as D-fructose to fructose 6-phosphate, contributing to hexose metabolism.
• Localizes to the mitochondrial outer membrane and cytosol; mitochondrial localization couples glycolysis to oxidative phosphorylation and prevents apoptosis by inhibiting release of apoptogenic molecules. Related references: PMID:18350175
• Predominant hexokinase isozyme in insulin-responsive tissues like skeletal muscle, linking energy sensing to glucose utilization.
• Contains allosteric sites for glucose 6-phosphate feedback inhibition and ATP-binding domains (UniProt keywords: Allosteric enzyme, ATP-binding, Kinase, Transferase).
• Subject to acetylation (UniProt keyword: Acetylation) and other post-translational modifications that may regulate activity and localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-120 amino acids of human Hexokinase II; this region may confer high specificity for the full-length protein.
• For WB, consider preparing mitochondrial fractions or whole-cell lysates from insulin-responsive tissues (e.g., skeletal muscle) to enrich for Hexokinase II.
• ELISA protocols may require optimization of coating antigen and detection conditions.
• Cross-reactivity with mouse and rat has been reported; validate detection in relevant cell lines or tissues.
• The predicted molecular weight is 102 kDa; confirm band size using appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This Hexokinase II monoclonal antibody supports research and assay development in glycolysis, diabetes, and apoptosis, helping ensure reliable performance for diagnostic manufacturers and research labs. Contact us for tailored supply solutions.