[KO Validated] Anti-Hexokinase-1 (HK1) Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - P19367

Core Product Specifications and Parameters

Biological Background: Hexokinase-1 Function and Localization

• Hexokinase-1 (HK1, also known as brain form hexokinase, HK I, HK-A) is a ~102 kDa glycolytic enzyme that catalyzes the phosphorylation of hexoses, primarily glucose, to produce glucose-6-phosphate in the initial step of glycolysis.
• HK1 is essential for glucose metabolism, phosphorylating D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, but not N-acetyl-D-glucosamine (PubMed:1637300, PubMed:25316723, PubMed:27374331). Related references: PMID:1637300 PMID:25316723 PMID:27374331
• Beyond its metabolic role, HK1 acts as a pattern recognition receptor for bacterial peptidoglycan; cytosolic N-acetyl-D-glucosamine from peptidoglycan inhibits its hexokinase activity and triggers dissociation from the mitochondrial outer membrane, leading to NLRP3 inflammasome activation and innate immune responses (PubMed:27374331). Related references: PMID:27374331
• HK1 localizes predominantly to the mitochondrial outer membrane, with a smaller pool in the cytosol. This tethering couples glycolysis to mitochondrial ATP production and influences metabolic signaling.
• Multiple isoforms of HK1 exist via alternative splicing: isoform 1 is widely expressed, isoform 2 is erythrocyte-specific, and isoforms 3 and 4 are testis-specific (PubMed:10978502). Related references: PMID:10978502
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and subcellular localization. HK1 is also an allosteric enzyme and an ATP-binding kinase.
• Mutations in HK1 are associated with diseases such as Charcot-Marie-Tooth disease, hereditary hemolytic anemia, intellectual disability, and retinitis pigmentosa, highlighting its critical physiological importance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50–150 of human Hexokinase-1, a region that may be conserved across species. Users working with mouse or rat samples should verify cross-reactivity in their model system.
• For Western blotting, a band near 102 kDa is expected; consider using reducing conditions and appropriate loading controls for mitochondrial proteins.
• In immunoprecipitation and immunofluorescence applications, keep in mind that HK1 is primarily localized to the mitochondrial outer membrane; permeabilization and organelle enrichment may enhance detection.
• For immunohistochemistry (IHC), antigen retrieval conditions and fixation methods should be optimized for mitochondrial antigens. Titrate the antibody in relevant tissue types.
• The antibody is suitable for ELISA; when designing quantitative assays, include appropriate standards and validate linearity and sensitivity in the specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated antibody raw materials such as this anti-HK1 monoclonal antibody, supporting glycolysis research, metabolic disorders, and innate immunity studies. As a trusted one-stop partner, we offer custom antibody development, bulk reagent supply, and technical consulting—streamlining your IVD workflow from concept to clinic. Our portfolio includes optimized monoclonal and polyclonal antibodies, matched antibody pairs, and ancillary reagents to accelerate your assay development.