[KO Validated] Anti-HDAC1 Rabbit Monoclonal Antibody for WB, IP, IF, IHC, ChIP, ELISA - Q13547

Core Product Specifications and Parameters

Biological Background: HDAC1 Function and Localization

• HDAC1 is a histone deacetylase that catalyzes the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4), leading to epigenetic repression and playing key roles in transcriptional regulation, cell cycle progression, and development (PubMed:16762839, PubMed:17704056, PubMed:28497810). Related references: PMID:16762839 PMID:17704056 PMID:28497810

• It functions within large multiprotein complexes, including the NuRD complex for chromatin remodeling (PubMed:16428440, PubMed:28977666) and the SIN3B complex, where it is recruited downstream of transcriptional start sites to modulate histone acetylation and RNA polymerase II progression (PubMed:21041482). Related references: PMID:16428440 PMID:28977666 PMID:21041482

• Beyond histones, HDAC1 deacetylates non-histone targets including SP1, SP3, RELA (NF-κB subunit), STAT3, NR1D2, and TSHZ3, thereby regulating transcription factor activity (PubMed:12837748, PubMed:16285960, PubMed:16337145, PubMed:16478997, PubMed:17000776, PubMed:17996965, PubMed:19343227). Related references: PMID:12837748 PMID:16285960 PMID:16337145 PMID:16478997 PMID:17000776 PMID:17996965 PMID:19343227

• In resting neurons, HDAC1 is part of the BRG1-RB1-HDAC1 complex that represses CREST-mediated transcription; upon calcium signaling, HDAC1 release allows CREBBP recruitment and transcriptional activation (PubMed:19081374). Related references: PMID:19081374

• HDAC1 localizes predominantly to the nucleus.

• It is ubiquitously expressed, with higher levels in heart, pancreas, and testis, and lower levels in kidney and brain.

• HDAC1 also possesses protein decrotonylase and delactylase activities, mediating removal of crotonyl and lactoyl modifications from histones (PubMed:28497810, PubMed:35044827). Related references: PMID:28497810 PMID:35044827

Experimental Guidance and Technical Tips

• For western blot (WB), use HDAC1-positive cell lysates (e.g., HeLa or HEK293T) and expect a band at ~55 kDa. Optimize antibody dilution based on sample type.
• For immunoprecipitation (IP), include an appropriate IgG control to confirm specificity; this antibody has been validated for IP in human and mouse samples.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), HDAC1 shows nuclear staining; include DAPI counterstain and optimize antigen retrieval for paraffin sections.
• For chromatin immunoprecipitation (ChIP), crosslinking and sonication conditions should be optimized for your chromatin preparation; this antibody is ChIP-suitable as per specifications.
• The antibody can be used in ELISA; validate employing recombinant HDAC1 or HDAC1-derived peptide antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs with high-quality reagents from discovery to clinical application. This HDAC1 antibody enhances epigenetics and gene regulation research, and we offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact CamelBio to streamline your raw material procurement.