[KO Validated] Anti-GLOD4 Polyclonal Antibody for WB, ELISA - Q9HC38

Core Product Specifications and Parameters

Biological Background: GLOD4 Function and Localization

• GLOD4 (Glyoxalase domain-containing protein 4), also known as HC6, HC71, CGI-150, C17orf25, or D4, is a human protein encoded by the GLOD4 gene (GeneID 51031).
• The protein features a glyoxalase domain, as indicated by its name and sequence analysis.
• Subcellular localization: Predominantly localized to the mitochondrion.
• Tissue specificity: Expressed in heart, brain, liver, kidney, pancreas, and placenta, but not in skeletal muscle or lung.
• GLOD4 is a phosphoprotein, with phosphorylation sites characterized via proteomics.
• Alternative splicing generates multiple transcript variants, contributing to potential isoform diversity.
• The three-dimensional structure of GLOD4 has been solved, providing insights into its domain architecture (UniProt keyword: 3D-structure).
• The predicted molecular weight of GLOD4 is approximately 35 kDa, consistent with the UniProt-calculated mass of 34.8 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to amino acids 179–298 of human GLOD4; therefore, it is likely to recognize the C-terminal region.
• For Western blot applications, a band migrating at approximately 35 kDa is expected; validate using a mitochondrial protein marker or fractionation controls.
• Pre-adsorption with the immunogen can serve as a specificity control.
• For ELISA, titrate the antibody against recombinant GLOD4 protein or tissue lysates to establish optimal working concentrations.
• Since GLOD4 expresses differentially across tissues, consider including positive (e.g., heart, liver) and negative (e.g., skeletal muscle) control samples in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply a wide range of IVD raw materials, including validated polyclonal antibodies like this anti-GLOD4 antibody for mitochondrial protein studies. Beyond single reagents, we offer bulk provision, ancillary reagents, and technical sourcing expertise to streamline your assay development from concept to clinic.