KO Validated Anti-GIT1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2X7

Core Product Specifications and Parameters

Biological Background: GIT1 Function and Localization

• GIT1 (ARF GTPase-activating protein GIT1), also known as p95-APP1 and CAT1, is a multidomain scaffold protein that participates in receptor internalization, focal adhesion remodeling, and signaling by G protein-coupled receptors and tyrosine kinase receptors.
• Through PAK1 activation, GIT1 positively regulates microtubule nucleation during interphase, and plays a role in cytokinesis via a pathway involving ENTR1 and PTPN13.
  Related references:
  PMID:27012601, PMID:23108400
• GIT1 promotes cell motility by regulating focal complex dynamics and locally activating RAC1. It also recruits MAPK1/3/ERK1/2 to focal adhesions after EGF stimulation, stimulating cell migration.
  Related references:
  PMID:10938112, PMID:11896197, PMID:15923189
• GIT1 is crucial for dendritic spine morphogenesis and synapse formation. In hippocampal neurons, it recruits ARHGEF7/beta-PIX to locally activate RAC1, essential for spine morphogenesis and synapse formation. It also regulates GABA(A) receptor synaptic stability by coordinating RAC1 and PAK1 activity, leading to F-actin stabilization and maintaining inhibitory synaptic transmission.
  Related references:
  PMID:12695502, PMID:15800193, PMID:25284783
• GIT1 is essential for VEGF signaling through activation of phospholipase C-gamma and ERK1/2, controlling endothelial cell proliferation and angiogenesis. It is also required for bone regeneration, partly through regulation of NF-kappa-B signaling in mesenchymal stem cells to enhance VEGFA production.
  Related references:
  PMID:19273721, PMID:31502302
• Subcellular localization: GIT1 is found in the cytoplasm, synapse (pre- and postsynaptic), postsynaptic density, focal adhesion, lamellipodium, centrosome, and spindle pole.
• Structural features: Contains ANK repeats, a coiled-coil domain, and a zinc-finger; undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 460–640 of human GIT1, a central region that may recognize conserved epitopes across known isoforms and species.
• For Western blot, the predicted molecular weight is ~84 kDa; additional bands may appear due to alternative splicing or post-translational modifications. Validate the antibody in the relevant sample system.
• For immunofluorescence/ICC, consider fixation and permeabilization conditions suitable for cytoskeletal and focal adhesion structures; co-staining with markers for F-actin or focal adhesion proteins may be informative.
• ELISA applications may require optimization of coating antigen and antibody dilution to achieve the desired sensitivity and specificity.
• Cross-reactivity with mouse and rat GIT1 is supported by supplier data; however, endogenous expression levels and tissue-specific isoforms should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing platform for IVD raw materials, supporting every stage from concept to clinic. This anti-GIT1 polyclonal antibody targets a key scaffold protein involved in cell adhesion and neuroscience research, and CamelBio can facilitate procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for related signaling or adhesion targets. With a focus on streamlined supply, CamelBio helps accelerate your assay development and production timelines.