[KO Validated] Anti-GATA3 Rabbit Polyclonal Antibody for WB and ELISA - P23771

Core Product Specifications and Parameters

Biological Background: GATA3 Function and Localization

• GATA3 is a member of the GATA family of zinc-finger transcription factors that recognizes the consensus motif 5'-AGATAG-3'.
• It functions as a transcriptional activator essential for T-cell receptor alpha and delta gene regulation.
• Plays a critical role in the differentiation of T-helper 2 (Th2) cells during immune and inflammatory responses.
• Involved in IL33-mediated macrophage activation, coordinating transcriptional activation and UCP2-dependent metabolic reprogramming.
• Promotes the differentiation of inflammation-resolving alternatively activated macrophages upon tissue injury.
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Expressed in T-cells and endothelial cells.
• Post-translationally modified by phosphorylation; alternative splicing produces multiple isoforms; associated with deafness and disease variants.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-260) of human GATA3; consider validating the antibody's reactivity with the target isoform of interest in your specific assay system.
• For Western blotting, run protein samples under reducing conditions and include appropriate positive and negative controls, such as knockout or overexpressing cell lysates.
• For ELISA, optimize coating conditions and consider using a recombinant GATA3 protein as a standard.
• Given reported cross-reactivity with human, mouse, and rat, validate the antibody in the relevant species model to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined, one-stop channel for IVD raw materials. Our extensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets like transcription factors. For your GATA3-related immunoassay development and manufacturing needs, contact us to discuss custom solutions and reliable supply.