KO Validated Anti-GARS Monoclonal Antibody for WB, ELISA - P41250

Core Product Specifications and Parameters

Biological Background: GARS Function and Localization

• GARS encodes glycyl-tRNA synthetase, a class II aminoacyl-tRNA synthetase that catalyzes the ATP-dependent attachment of glycine to its cognate tRNA during protein synthesis. Related references: PMID:17544401 PMID:24898252 PMID:28675565
• In addition to its role in translation, GARS functions as a diadenosine tetraphosphate synthetase (Ap4A synthetase), producing the signaling molecule Ap4A by condensation of two ATP molecules, thereby contributing to cellular regulation pathways. Related references: PMID:19710017
• The protein localizes to the cytoplasm, and is also found in mitochondria, cell projections (axons), and can be secreted via extracellular exosomes.
• GARS is widely expressed, with documented presence in brain, spinal cord, muscle, heart, spleen, and liver.
• Mutations in GARS are associated with Charcot-Marie-Tooth disease type 2D and distal spinal muscular atrophy type V, reflecting its importance in neuronal function.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains an ATP-binding domain and a transit peptide for mitochondrial targeting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C-terminal region (amino acids 640–739) of human GARS; the antibody may recognize both full-length and truncated protein forms depending on sample context.
• For Western blot, an expected band around 83 kDa is anticipated in human samples; consider using appropriate positive controls and molecular weight markers.
• The antibody is validated for WB and ELISA; for ELISA, direct or sandwich formats using the immunogen peptide as standard may be explored.
• Cross-reactivity is reported for human; performance with other species has not been characterized and should be validated empirically if needed.
• Storage and handling: shipped on ice bags; upon receipt, aliquot and store at recommended temperatures to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, technical services, and consulting support—from concept to clinic. This anti-GARS monoclonal antibody exemplifies our portfolio for neuromuscular and neurodegenerative disease research, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare target raw materials. Let us streamline your procurement needs so you can focus on advancing your assays and discoveries.