[KO Validated] Anti-Galectin-3/LGALS3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3 is a galactose-specific lectin that binds IgE and mediates various cell-cell and cell-matrix interactions, including integrin-mediated endothelial cell migration.
• In the cytoplasm, it participates in acute inflammatory responses such as neutrophil activation, monocyte/macrophage chemoattraction, and mast cell activation.
• Together with TRIM16, Galectin-3 coordinates membrane damage recognition and mobilizes autophagy regulators ATG16L1 and BECN1 to damaged endomembranes.
• In the nucleus, Galectin-3 acts as a pre-mRNA splicing factor, contributing to mRNA processing.
• The protein localizes to the cytoplasm, nucleus, and is also secreted, functioning in both intracellular and extracellular environments.
• Major expression is observed in the colonic epithelium, activated macrophages, and fetal membranes.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 26 kDa. Consider using reducing conditions to address potential disulfide bond formation.
• In immunofluorescence/immunocytochemistry, optimize permeabilization protocols to allow detection of nuclear and cytoplasmic pools of Galectin-3.
• For ELISA, the antibody may be used to detect both native and recombinant Galectin-3; validate in the relevant sample matrix.
• The polyclonal nature of this antibody can provide robust signal for detecting multiple epitopes; confirm specificity using appropriate positive and negative controls.
• With confirmed cross-reactivity to mouse and rat, this antibody is suitable for translational studies in common rodent models; validate species-specific reactivity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This Galectin-3 antibody, targeting a key mediator of inflammation and cellular homeostasis, is part of our extensive offering for oncological and immunological assay development. Our sourcing experts support you from concept to clinic, enabling rapid procurement of rare target raw materials and customized technical consulting.