[KO Validated] Anti-FAM38A/PIEZO1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92508

Core Product Specifications and Parameters

Biological Background: PIEZO1 Function and Localization

• PIEZO1 is the pore-forming subunit of a mechanosensitive non-selective cation channel that mediates rapidly adapting mechanically activated (MA) currents, essential for sensing touch and tactile pain.
  Related references: PMID:23479567, PMID:23695678, PMID:25955826
• The channel functions as a homotrimer with a three-blade propeller structure, gated by a cap-motion and plug-and-latch mechanism.
  Related references: PMID:37590348
• PIEZO1 exhibits a linear current-voltage relationship, with conductance to monovalent ions following K⁺ > Na⁺ > Li⁺; divalent ions permeate more slowly and reduce K⁺ currents.
  Related references: PMID:25955826
• Beyond mechanosensation, PIEZO1 maintains epithelial cell adhesion by promoting integrin activation through R-Ras/calpain signaling at the endoplasmic reticulum.
  Related references: PMID:20016066
• Acts as a shear-stress sensor in endothelial cells, promoting cell alignment in the direction of blood flow via calpain activation, which is crucial for vascular development and physiology.
  Related references: PMID:25119035
• Senses phosphatidylserine flipping at the plasma membrane, triggering Ca²⁺ influx and Rho GTPase signaling that drives myoblast fusion and muscle cell morphogenesis.
  Related references: PMID:29799007
• Localizes to the endoplasmic reticulum, ER-Golgi intermediate compartment, and cell membrane, with enrichment in lamellipodia at the leading edge of migrating cells.
• Widely expressed in tissues, including neurons, erythrocytes, and myoblasts; in Alzheimer disease, detected in activated astrocytes surrounding senile plaques, but not in normal astrocytes.

Experimental Guidance and Technical Tips

• For Western blotting, note the large molecular weight (~287 kDa); use low-percentage SDS-PAGE gels (e.g., 6% resolving gel) and extended transfer times with wet transfer systems for efficient blotting.
• The immunogen (amino acids 2199–2429) targets a C-terminal region; verify that any splice variants of interest contain this epitope.
• The antibody is KO-validated; include knockout cell lysates as negative controls in your WB experiments to confirm specificity.
• For IHC-P, optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) and include isotype controls to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality antibodies, including this anti-PIEZO1 monoclonal antibody, alongside validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. Whether you are advancing mechanobiology, vascular research, or Alzheimer's disease diagnostics, our comprehensive supply chain ensures reliable, one-stop procurement from concept to clinic.