[KO Validated] Anti-ESD Rabbit Polyclonal Antibody for WB and ELISA - P10768

Core Product Specifications and Parameters

Biological Background: ESD Function and Localization

• ESD (S-formylglutathione hydrolase, also known as Esterase D or Methylumbelliferyl-acetate deacetylase) is a serine hydrolase encoded by the ESD gene (Gene ID: 2098).
• It catalyzes the hydrolysis of S-formylglutathione to form glutathione and formate, playing a central role in formaldehyde detoxification.
• ESD is primarily localized in the cytoplasm and cytoplasmic vesicles.
• As a member of the serine esterase family, it functions as a hydrolase.
• Post-translational modifications include acetylation, and its three-dimensional structure has been experimentally determined.
• The protein has been identified via direct protein sequencing and large-scale proteomics studies.
• The calculated molecular weight is approximately 31.5 kDa, consistent with the observed band near 31 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans nearly full-length human ESD (aa 1–282), likely eliciting polyclonal antibodies that recognize multiple epitopes.
• For Western blot, a band around 31 kDa is expected in lysates from human or mouse cells/tissues; KO validation is recommended to confirm specificity.
• In ELISA, this antibody may be used as a capture or detection reagent, but optimal pairing and dilution should be determined empirically.
• Cross-reactivity with mouse is confirmed; for other species, sequence homology should be checked if needed.
• Consider optimizing blocking and washing conditions to minimize background, as with any polyclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies, monoclonal antibodies, antigen pairs, and ancillary reagents. This anti-ESD antibody serves as a reliable tool for studying formaldehyde metabolism and esterase D function, complementing our offerings in rare-target sourcing and bulk supply. Our consultative approach helps bridge the gap from early-stage development to clinical application.