[KO Validated] Anti-ErbB3/HER3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3 (HER3) is a receptor tyrosine-protein kinase encoded by the ERBB3 gene, also known as proto-oncogene-like protein c-ErbB-3 and tyrosine kinase-type cell surface receptor HER3. Multiple isoforms exist due to alternative splicing (e.g., erbB3-S, p45-sErbB3, p85-sErbB3).
• Functions as a cell surface receptor for neuregulins, particularly neuregulin-1 (NRG1). Ligand binding induces tyrosine phosphorylation and promotes association with the p85 subunit of phosphatidylinositol 3-kinase (PI3K), initiating downstream signaling pathways. Related references: PMID:20682778
• May also be activated by chondroitin sulfate proteoglycan 5 (CSPG5), suggesting alternative modes of receptor engagement. Related references: PMID:15358134
• Involved in the regulation of myeloid cell differentiation, highlighting its role beyond epithelial cells. Related references: PMID:27416908
• Subcellularly located primarily at the cell membrane, with a secreted form also detected, consistent with the presence of a signal peptide and transmembrane domain.
• Highly expressed in epithelial tissues and brain, aligning with its roles in development and cancer.
• Classified under multiple UniProt keywords including ATP-binding, kinase, phosphoprotein, and disease variant, indicating its involvement in phosphorylation-dependent signaling and cancer susceptibility.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to the C-terminus (aa 1275–1342) of human ErbB3. This region is common to full-length and certain secreted isoforms, so the antibody may detect multiple bands in WB.
• For WB, a band near 148 kDa is expected for the full-length receptor; consider reducing conditions and appropriate molecular weight markers.
• For IF/ICC, fixation and permeabilization may be required to access the intracellular epitope; validate localization in the appropriate cell line.
• KO validation indicates that the antibody exhibits reduced signal in ERBB3 knockout cells; consider using KO samples as negative controls to confirm specificity in your assay.
• Since it is a polyclonal antibody, lot-to-lot variability may exist; performance should be verified in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for diagnostic manufacturers and research laboratories. This anti-ErbB3/HER3 antibody supports cancer biology and epithelial tissue studies, and we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your assay development.