[KO Validated] Anti-EEF1B2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P24534

Core Product Specifications and Parameters

Biological Background: EEF1B2 Function and Localization

• EEF1B2 encodes Elongation factor 1-beta (also known as eEF-1B alpha or EF-1-beta-alpha), a core component of the eukaryotic elongation factor 1 (eEF1) complex.
• Functions as the catalytic subunit of the eEF1B guanine nucleotide exchange factor (GEF) subcomplex, which stimulates GDP release and GTP binding on elongation factor 1A (eEF1A) to drive translational elongation.
• Plays a critical role in protein biosynthesis by regenerating active eEF1A•GTP, thereby enabling codon-anticodon decoding and ribosome translocation.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity and interaction with other eEF1 subunits and the ribosome.
• Contains isopeptide bonds and is subject to ubiquitin conjugation, suggesting involvement in protein turnover or signaling complex assembly.
• Identified in proteomics screenings and supported by direct protein sequencing (UniProt), indicating its expression across human tissues.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the N-terminal 225 amino acids, so the antibody likely detects the full-length EEF1B2 protein at ~25 kDa in WB. Consider using whole cell lysates from HeLa or HEK293T as positive controls.
• For IHC-P and IF/ICC, fixation and permeabilization may affect epitope accessibility; test with 4% PFA fixation and Triton X-100 permeabilization.
• ELISA: this antibody can be used in sandwich ELISA or direct detection, but optimal concentrations should be determined by checkerboard titration.
• KO validation: the antibody has been knockout-validated, confirming specificity; run alongside a wild-type control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing of IVD raw materials, including this anti-EEF1B2 polyclonal antibody, supporting research in protein translation and biosynthesis. We offer validated antibody pairs, bulk reagents, and customized sourcing for rare targets, helping diagnostic and research customers streamline development from concept to clinic.