[KO Validated] Anti-ECE1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42892

Core Product Specifications and Parameters

Biological Background: ECE1 Function and Localization

• Endothelin-converting enzyme 1 (ECE1) is a zinc-dependent metalloprotease that catalyzes the conversion of big endothelin-1 to endothelin-1, a potent vasoactive peptide.
• ECE1 is a single-pass type II membrane protein localized primarily to the cell membrane, where it exerts its enzymatic activity.
• Alternative splicing generates multiple isoforms (A, B, C, D) that exhibit tissue-specific expression patterns, including in endothelial cells, heart, placenta, and kidney.
• The enzyme is implicated in cardiovascular homeostasis and pathogenesis, with roles in blood pressure regulation and vascular remodeling.
• Post-translational modifications include N-glycosylation and phosphorylation, which may modulate enzyme activity and stability.
• Mutations in the ECE1 gene are linked to Hirschsprung disease, highlighting its importance in enteric nervous system development.
• UniProt annotates ECE1 with keywords such as signal-anchor, disulfide bond, and disease variant, indicating structural features and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 670–770) of human ECE1, which shares high homology across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blotting, a band at approximately 87 kDa is expected; however, isoform variation and post-translational modifications may cause shifts in migration. Consider optimizing reducing conditions.
• In immunohistochemistry (IHC-P), antigen retrieval is recommended due to the transmembrane nature of the target. Use appropriate tissue controls expressing ECE1, such as kidney or heart.
• For immunofluorescence (IF/ICC), permeabilization is required to access the intracellular epitope. Validate staining localization at the cell membrane.
• When setting up ELISA, pair with a well-characterized capture antibody and use a recombinant ECE1 standard for quantitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. In the field of cardiovascular and endothelin pathway research, we support projects from concept to clinical application with optimized antibody products and rare target sourcing. Contact us to discuss how CamelBio can accelerate your ECE1-related assay development and biomarker studies.