KO Validated Anti-DNAJB1 Polyclonal Antibody for WB, IF/ICC, ELISA - P25685

Core Product Specifications and Parameters

Biological Background: DNAJB1 Function and Localization

• DNAJB1 (DnaJ homolog subfamily B member 1), also known as Hdj1, HSP40, HSPF1, or Sis1, is a member of the DnaJ/HSP40 chaperone family. Alternative splicing may generate multiple isoforms.
• It functions as a co-chaperone that interacts with HSP70, stimulating its ATPase activity to promote protein folding. Related references: PMID:14503850
• Together with TTC1, DNAJB1 acts as a chaperone adapter, regulating HSP70-dependent folding by binding the HSP70 amino‑terminal region.
• DNAJB1 negatively regulates heat shock‑induced HSF1 transcriptional activity during the attenuation and recovery phases of the heat shock response. Related references: PMID:9499401
• It stimulates ATP hydrolysis and the refolding of denatured proteins by HSPA1A/B in vitro. Related references: PMID:24318877
• Subcellular localization includes the cytoplasm, nucleus, and nucleolus, reflecting its roles in both chaperone‑mediated folding and stress‑responsive transcriptional regulation.
• DNAJB1 is a phosphoprotein, with post‑translational modifications potentially modulating its activity and interactions.
• As a stress‑inducible chaperone, DNAJB1 is critical for cellular proteostasis, particularly under conditions that challenge protein folding homeostasis.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1‑340 of human DNAJB1, covering the full‑length protein, which may enable recognition of multiple isoforms and conserved epitopes.
• For western blotting (WB), the predicted molecular weight of DNAJB1 is approximately 38 kDa; consider using a suitable loading control and verifying band specificity with knockout‑validated lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect both cytoplasmic and nuclear/nucleolar staining patterns; optimize fixation and permeabilization conditions to preserve epitope accessibility.
• When performing ELISA, titrate the antibody to determine optimal coating and detection concentrations; validate reactivity with recombinant DNAJB1 or cell lysates from human, mouse, or rat.
• Because DNAJB1 is a stress‑inducible protein, expression levels may vary; induce heat shock or other stressors in your model system to enhance signal if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, including this high‑quality DNAJB1 antibody. With a focus on chaperone and stress response targets, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare‑target raw materials to support your assay development from concept to clinic. Partner with CamelBio to streamline your procurement and accelerate your diagnostic projects.