[KO Validated] Anti-DJ-1/PARK7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99497

Core Product Specifications and Parameters

Biological Background: DJ-1/PARK7 Function and Localization

• DJ-1/PARK7, also known as Maillard deglycase, oncogene DJ1, and protein/nucleic acid deglycase DJ-1 (aliases: DJ1, GATD2, HEL-S-67p, K7), is a multifunctional protein implicated in cellular protection.
• Core oxidative stress sensor: acts as a redox-sensitive chaperone and protease, protecting cells against oxidative stress and cell death. Related references: PMID:12796482 PMID:17015834 PMID:18711745
• Protein and nucleotide deglycase activity: repairs methylglyoxal- and glyoxal-glycated adducts on proteins, nucleotides, and histones, thereby maintaining chromatin architecture; this function is debated. Related references: PMID:25416785 PMID:28596309 PMID:30150385 PMID:30894531 PMID:26995087 PMID:28013050 PMID:27903648 PMID:31653696
• Neuroprotective roles: stabilizes NFE2L2 and PINK1, modulates NF-kappa-B signaling, and supports cell growth and transformation. Related references: PMID:12612053 PMID:14749723 PMID:15502874
• Hydrogen peroxide detoxification: eliminates hydrogen peroxide, protecting cells against hydrogen peroxide-induced death. Related references: PMID:16390825
• Mitochondrial homeostasis: required for correct mitochondrial morphology and autophagic degradation of dysfunctional mitochondria. Related references: PMID:16632486 PMID:19229105
• Subcellular localization: present in the cell membrane, cytoplasm, nucleus, membrane rafts, mitochondria, and endoplasmic reticulum.
• Tissue specificity: highly expressed in pancreas, kidney, skeletal muscle, liver, testis, and heart; lower levels in placenta and brain; detected in astrocytes, Sertoli cells, spermatogonia, spermatids, and spermatozoa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-189) of human DJ-1/PARK7; consider its potential to recognize full-length and possibly cleaved forms of the protein. Validate specificity using knockout or knockdown models where available.
• For western blotting (WB), a band of approximately 20 kDa is expected. Include appropriate positive controls such as lysates from HeLa, MCF7, or mouse brain tissue (based on cross-reactivity with human and mouse).
• For immunofluorescence/immunocytochemistry (IF/ICC), anticipate staining patterns consistent with cytoplasmic, nuclear, and mitochondrial localization. Use relevant cell lines; perform appropriate controls (e.g., peptide blocking) to confirm specificity.
• For ELISA, the polyclonal nature may offer high sensitivity; however, optimize coating buffers and detection conditions for the target antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories as a comprehensive one-stop source for IVD raw materials. For targets like DJ-1/PARK7, relevant to oxidative stress, neurodegeneration, and oncology, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing. Let CamelBio bridge your concept-to-clinic journey with reliable, quality-controlled immunoreagents.