KO Validated Anti-DDIT4 Polyclonal Antibody for WB, ELISA - Q9NX09

Core Product Specifications and Parameters

Biological Background: DDIT4 Function and Localization

• DNA damage-inducible transcript 4 protein (DDIT4), also known as REDD1, RTP801, Dig2, and REDD-1, is a 25 kDa protein involved in cellular stress responses.
• Functions as a negative regulator of mTORC1, inhibiting its activity through a pathway that involves AKT1, the TSC1-TSC2 complex, and the GTPase RHEB, thereby controlling cell growth and proliferation.
• Mediates cellular responses to hypoxia, DNA damage, and energy depletion; its role in hypoxia-dependent mTORC1 inhibition is cell-type specific, occurring in fibroblasts and thymocytes but not hepatocytes.
• Plays a role in p53/TP53-mediated apoptosis upon DNA damage by modulating mTORC1 activity, and is additionally required for antiviral defense by restricting viral protein synthesis.
• Localizes to the mitochondrion and cytoplasm (primarily cytosol), consistent with its role in metabolic and stress signaling.
• Broadly expressed across tissues with lower levels in brain, skeletal muscle, and intestine; protein levels are upregulated in substantia nigra neurons from Parkinson disease patients.
• Subject to post-translational modifications including phosphorylation and ubiquitination (Ubl conjugation), as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human DDIT4 protein (aa 1-232), potentially generating antibodies against multiple epitopes. Consider validating specificity in your sample system using knockout controls.
• For Western blot, a band around 25 kDa is expected under reducing conditions. Optimize sample preparation and loading amounts to achieve clear detection.
• In ELISA, the antibody can be used for capture or detection of recombinant or endogenous DDIT4. Validate cross-reactivity and sensitivity with appropriate standards.
• As a polyclonal antibody, lot-to-lot variation may occur. Assess performance consistency when changing batches.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines your procurement of validated antibodies, antibody pairs, and bulk ancillary reagents for diagnostic and research workflows. Our portfolio includes this DDIT4 polyclonal antibody to support mTOR pathway and stress response investigations, alongside custom sourcing capabilities for rare or hard-to-find targets. Rely on CamelBio to bridge the gap from concept to clinic with reliable supply and expert support.