KO Validated Anti-Cytokeratin 8/KRT8 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8/KRT8 Function and Localization

• Keratin 8 (KRT8) is a type II intermediate filament protein that obligately pairs with the type I keratin KRT18 to form the keratin cytoskeleton in simple epithelial cells.
• The KRT8/KRT18 network provides mechanical stability and resilience to epithelial cells, enabling them to withstand physical stress. Related references: PMID:26823019
• KRT8 protects epithelial cells from non-mechanical stress, including apoptosis, contributing to epithelial integrity.
• Through ARHGEF40, KRT8 mediates actin stress fiber formation and is required for tensional force-induced stress fiber reinforcement. Related references: PMID:26823019
• In striated muscle, KRT8, together with KRT19, may link the contractile apparatus to dystrophin at costameres. Related references: PMID:16000376
• Subcellular localization includes the cytoplasm, nucleoplasm, and nuclear matrix, reflecting dynamic roles in cytoskeletal and nuclear organization.
• Post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination regulate KRT8 function and filament dynamics.
• Alternatively spliced isoforms and modifications account for observed protein weights of approximately 54 and 56 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved sequence within amino acids 341–440 of human KRT8; this region may be critical for detection across species and isoforms.
• For Western blot (WB), a protein band around 54–56 kDa is expected; verify molecular weight markers and consider using a 10% SDS-PAGE gel for optimal resolution.
• For immunohistochemistry (IHC-P), antigen retrieval using heat-induced epitope retrieval (HIER) or enzymatic methods is recommended due to the filamentous nature of keratins.
• The antibody cross-reacts with rat, allowing its use in rodent tissue models; validate in the relevant sample system to confirm performance.
• As a rabbit monoclonal antibody, it likely offers high affinity and batch-to-batch consistency; titration experiments are advised for new applications.

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CamelBio provides diagnostic manufacturers and research labs with a one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects targeting epithelial biology and cancer research, we offer streamlined access to rare target raw materials and technical consultation. Our integrated sourcing bridges the gap from concept to clinic, supporting your development of robust diagnostic assays.