[KO Validated] Anti-Cytokeratin 20 (KRT20) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P35900

Core Product Specifications and Parameters

Biological Background: Cytokeratin 20 Function and Localization

• Cytokeratin 20 (KRT20), also known as CK-20, K20, or Protein IT, is a type I keratin intermediate filament protein that forms heterodimers with type II keratins to assemble the cytoskeletal network in epithelial cells.
• It plays a critical role in maintaining keratin filament organization specifically in intestinal epithelia, contributing to cellular integrity and mechanical resilience.
• Upon phosphorylation, KRT20 participates in the regulation of mucin secretion in the small intestine, linking keratin dynamics to secretory functions in the gut.
• The protein is encoded by the KRT20 gene and belongs to the large keratin gene family, which includes multiple tissue-specific isoforms.
• Subcellularly, KRT20 localizes to the cytoplasm, where it integrates into the intermediate filament network.
• Expression is predominantly restricted to the intestinal epithelium, including luminal cells of colonic mucosa, but it is also detected in Merkel cells of keratinized oral mucosa (gingival, palatal, and tongue taste buds).
• Post-translational modifications, particularly phosphorylation, modulate its function; the protein is classified as a phosphoprotein and is associated with apoptosis, coiled-coil structures, and reference proteome identification.

Experimental Guidance and Technical Tips

• For WB analysis, consider using whole-cell lysates from intestinal epithelial cell lines such as Caco-2 or HT-29, or tissue homogenates from colon or small intestine. Validate the antibody in your specific sample matrix, as signal intensity may vary.
• In IF/ICC, ensure adequate permeabilization to allow antibody access to the cytoplasmic intermediate filament network. Co-staining with other epithelial markers (e.g., E-cadherin) can help confirm tissue context.
• Because the immunogen spans amino acids 245–424 of the central rod domain, the antibody may recognize both phosphorylated and unphosphorylated forms. For phosphorylation-dependent studies, orthogonal validation is recommended.
• The reported cross-reactivity with mouse and rat suggests utility in common model organisms; however, verify species-specific reactivity in your experimental setup, especially for non-human tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-KRT20 reagent, bulk ancillary reagents, and sourcing for rare targets. Our one-stop platform supports projects from concept to clinic, enabling efficient procurement for keratin biology, gastrointestinal disease research, and beyond. Contact us to discuss your specific supply needs and technical requirements.