KO Validated Anti-CYPOR (POR) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16435

Core Product Specifications and Parameters

Biological Background: NADPH--cytochrome P450 reductase Function and Localization

• POR (CYPOR) encodes NADPH--cytochrome P450 reductase, a membrane-bound flavoprotein essential for microsomal electron transport.
• It transfers electrons from NADP to cytochrome P450 enzymes, enabling monooxygenase reactions critical for drug metabolism, steroidogenesis, and detoxification.
• The enzyme also donates electrons to heme oxygenase and cytochrome B5, expanding its role in heme catabolism and redox balance.
• Contains FAD and FMN cofactors (flavoprotein), characteristic of the diflavin reductase family.
• Localized to the endoplasmic reticulum membrane via an N-terminal transmembrane helix; the catalytic domain faces the cytosol.
• Post‑translational modifications include acetylation and phosphorylation, potentially regulating its activity or interactions.
• Associated with human diseases: congenital adrenal hyperplasia and Antley‑Bixler syndrome (craniosynostosis), based on UniProt keywords.
• The calculated molecular weight is approximately 76.7 kDa, with the product detecting a band around 77 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises the N‑terminal region (aa 1–250), which may influence epitope accessibility in denaturing vs. native conditions; consider testing for specific applications.
• For western blotting, the expected band is ~77 kDa; use a suitable molecular weight marker for confirmation.
• KO validation (as indicated) supports specificity; verify in your own model system to ensure performance.
• In IHC‑P and IF/ICC, antigen retrieval and permeabilization may be required; optimize fixation and antibody dilution for your samples.
• The antibody cross‑reacts with mouse and rat CYPOR, enabling multi‑species studies; validate species‑specific expression patterns.
• Polyclonal format may yield batch‑to‑batch variability; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target procurement — all supported by technical consultation. This anti‑CYPOR (POR) antibody targets a key player in cytochrome P450 biology, relevant for drug metabolism and oxidoreductase research. Let our supply chain expertise accelerate your assay development and diagnostic workflows.