[KO Validated] Anti-Cyclin D1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P24385

Core Product Specifications and Parameters

Biological Background: Cyclin D1 Function and Localization

• Cyclin D1 (BCL1, PRAD1) is a G1/S-specific cyclin that, together with CDK4, phosphorylates and inhibits retinoblastoma (RB) proteins, releasing E2F transcription factors to promote G1/S transition. Related references: PMID:1827756, PMID:1833066, PMID:19412162
• The cyclin D1-CDK4 complex also phosphorylates SMAD3, repressing its transcriptional activity in a cell-cycle-dependent manner. Related references: PMID:15241418
• Cyclin D1 acts as a transcriptional corepressor with INSM1 on NEUROD1 and INS promoters, independently of cell cycle progression. Related references: PMID:16569215, PMID:18417529
• The protein localizes to the nucleus, cytoplasm, and nuclear membrane, consistent with its multiple regulatory roles.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond, Ubl conjugation), reflecting tight regulation of its activity and stability.
• Keywords from UniProt highlight its involvement in cell cycle, cell division, DNA damage, chromosomal rearrangement, and transcription regulation, underscoring its oncogenic potential.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 196–295 of human Cyclin D1 (P24385), a region that is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, consider using whole-cell lysates from proliferating cell lines (e.g., HeLa, MCF-7) where Cyclin D1 expression is high, and validate in your specific sample system.
• In IF/ICC, nuclear and cytoplasmic staining may be observed; optimize fixation and permeabilization for your cell type.
• ELISA performance should be assessed using recombinant Cyclin D1 or cell lysates as coating antigens; no specific dilution or protocol is provided.
• This antibody is KO validated; confirm endogenous target specificity by using appropriate knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined sourcing solution for IVD raw materials, including validated antibodies targeting cell cycle proteins like Cyclin D1. Our portfolio covers monoclonal and polyclonal antibodies, optimized pairs, bulk ancillary reagents, and access to rare targets, supporting everything from assay development to full-scale production.