KO Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody for WB and ELISA - P14635

Core Product Specifications and Parameters

Biological Background: Cyclin B1 Function and Localization

• G2/mitotic-specific cyclin-B1, encoded by the CCNB1 gene, is a key regulator of cell cycle progression.
• Essential for the control of the cell cycle at the G2/M (mitosis) transition. Related references: PMID:27030811 PMID:17495531 PMID:17495533
• Binds and activates cyclin-dependent protein kinase CDK1/CDC2, forming the M-phase promoting factor (MPF). Related references: PMID:41100585
• Localizes to the cytoplasm, nucleus, and centrosome during the cell cycle.
• Undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which regulate its activity and degradation.
• An alternatively spliced form exists, as indicated by UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 3–177), which may influence epitope accessibility.
• For Western blot, expect a band at approximately 48 kDa corresponding to Cyclin B1.
• Validate antibody reactivity in your specific experimental system, as cross‑reactivity has only been confirmed for human.
• This antibody is compatible with ELISA applications; optimize coating and detection conditions for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for cell cycle and oncology research. This Cyclin B1 monoclonal antibody exemplifies our commitment to providing high-quality, application-tested reagents to diagnostic manufacturers and research laboratories. Beyond standard catalog products, we also support custom sourcing for rare targets, helping you accelerate development from concept to clinic.