[KO Validated] Anti-CIRBP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14011

Core Product Specifications and Parameters

Biological Background: CIRBP Function and Localization

• Cold-inducible RNA-binding protein (CIRBP), also known as A18 hnRNP or Glycine-rich RNA-binding protein CIRP, is encoded by the CIRBP gene in humans.
• Plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival; acts as a translational activator.
• Essential for cold-induced suppression of cell proliferation, highlighting its role in cold-shock responses.
• Binds specifically to the 3’-untranslated regions (3’-UTRs) of stress-responsive transcripts RPA2 and TXN; functions as a translational repressor by similarity.
• Promotes assembly of stress granules (SGs) when overexpressed, participating in cellular stress granule dynamics.
• Localizes to the nucleus, nucleoplasm, and cytoplasm, enabling both transcriptional and cytoplasmic regulatory functions.
• Ubiquitously expressed across tissues, consistent with its fundamental role in stress responses.
• Classified as a phosphoprotein with RNA-binding activity, involved in alternative splicing and stress responses.

Experimental Guidance and Technical Tips

• For WB, consider using cold-shock or genotoxic stress-treated cell lysates to induce CIRBP expression; the predicted molecular weight is ~19 kDa.
• In IHC-P and IF/ICC, nuclear and cytoplasmic localization may be observed; antigen retrieval and permeabilization steps may be required and should be validated in target tissue/cell systems.
• As a polyclonal antibody, pre-adsorption against the immunogen may reduce non-specific background in certain applications.
• For ELISA, the antibody can be used as a capture or detection reagent; consider performing checkerboard titrations with complementary antibodies or recombinant proteins to optimize the assay.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, including validated antibodies like this CIRBP polyclonal for stress-response and cold-shock research.
• Beyond individual antibodies, we supply antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and can source rare targets to accelerate your assay development from concept to clinic.