[KO Validated] Anti-CHCHD2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6H1

Core Product Specifications and Parameters

Biological Background: CHCHD2 Function and Localization

• CHCHD2 (Coiled-coil-helix-coiled-coil-helix domain-containing protein 2), also referred to as Aging-associated gene 10 protein or HCV NS2 trans-regulated protein (NS2TP), is a transcriptional activator.
• It binds the oxygen responsive element (ORE) in the promoter of COX4I2 and stimulates its transcription under both hypoxic and normoxic conditions. Related references: PMID:23303788
• The protein localizes to the nucleus and mitochondria, including the mitochondrial intermembrane space, consistent with its dual roles in nuclear transcription and mitochondrial biology.
• Mutations in CHCHD2 are associated with Parkinson disease (PARK22) and neurodegeneration, underscoring its critical function in neuronal survival and mitochondrial integrity.
• This antibody targets amino acids 75–145 of human CHCHD2; the immunogen sequence is conserved in mouse and rat, supporting cross-reactivity.
• The full-length CHCHD2 has a predicted molecular weight of 15.5 kDa (UniProt Q9Y6H1), while the product specification lists 16 kDa, which may reflect slight differences in migration or modification.

Experimental Guidance and Technical Tips

• Given that the immunogen corresponds to amino acids 75–145, this antibody may detect the full-length CHCHD2 protein and potentially splice variants or processed forms. Validate in the relevant sample system.
• For Western blot (WB), a band around 15-16 kDa is expected; inclusion of appropriate positive controls (e.g., HeLa, HEK293) is recommended.
• For immunohistochemistry (IHC) and immunofluorescence (IF), consider testing both nuclear and mitochondrial staining patterns, as CHCHD2 localizes to both compartments.
• ELISA performance may vary; optimize coating antigen and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This CHCHD2 antibody supports research into mitochondrial biology and Parkinson's disease mechanisms. CamelBio offers a comprehensive portfolio of antibodies, including this polyclonal antibody, validated antibody pairs, and ancillary reagents, ensuring reliable supply for assay development.