[KO Validated] Anti-CETN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41208

Core Product Specifications and Parameters

Biological Background: CETN2 Function and Localization

• CETN2 encodes centrin-2 (also known as caltractin isoform 1), a calcium-binding protein of the centrin family.
• Essential for microtubule organizing center structure and function; required for centriole duplication and correct spindle formation during cell division.
• Regulates cytokinesis and contributes to genome stability via functional cooperation with CALM1 and CCP110.
• Serves as a component of the XPC complex in global genome nucleotide excision repair (GG-NER), where it helps stabilize XPC and stimulates its DNA-binding activity with RAD23B.
• Participates in mRNA export from the nucleus as part of the TREX-2 complex, facilitating translocation through nuclear pore complexes.
• Localizes to the centrosome, centriole, nuclear envelope, and nuclear pore complex, with additional cytoplasmic distribution.
• Subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, consistent with its roles in cell cycle and DNA repair regulation.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against full-length recombinant human CETN2 (aa 1-172), may detect multiple isoforms or modified forms; consider using appropriate controls to verify specificity in your system.
• For Western blot, expect a band at approximately 20 kDa (predicted molecular weight); optimize blocking and antibody dilution conditions for your samples.
• In immunofluorescence/ICC, centrosomal and nuclear pore staining may require optimized fixation and permeabilization protocols (e.g., methanol or paraformaldehyde with Triton X-100).
• Cross-reactivity with mouse and rat CETN2 is confirmed; for other species, check sequence homology before use.
• Include positive (e.g., HeLa or HEK293 whole-cell lysate) and negative (knockout or knockdown) controls to validate performance in your application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For centrosome biology, genome stability, and DNA repair research, we supply validated CETN2 polyclonal antibodies suitable for WB and IF/ICC, along with bulk ancillary reagents and custom sourcing services. From concept to clinic, CamelBio aggregates your raw-material needs to accelerate diagnostic development.