[KO Validated] Anti-CDKN2A/p16INK4a Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P42771 / Q8N726

Core Product Specifications and Parameters

Biological Background: CDKN2A/p16INK4a Function and Localization

• CDKN2A encodes the cyclin-dependent kinase inhibitor 2A, also known as p16INK4a, multiple tumor suppressor 1 (MTS-1), and CDK4I. It functions as a negative regulator of cell cycle progression by inhibiting CDK4 and CDK6, preventing phosphorylation of retinoblastoma protein.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in controlling cell cycle entry.
• CDKN2A is widely expressed in normal tissues, with exceptions including brain and skeletal muscle. Isoform 3 shows pancreas-specific expression.
• p16INK4a is a tumor suppressor frequently mutated or deleted in various cancers, including Li-Fraumeni syndrome, underscoring its critical role in maintaining genomic stability.
• The protein undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its activity and stability.
• p16INK4a contains ANK repeat motifs, facilitating protein-protein interactions essential for its inhibitory function on CDK4/6.
• Functional studies demonstrate that p16INK4a induction leads to cell cycle arrest in G1 phase, highlighting its importance in senescence and tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–156 of human p16INK4a (NP_000068.1). For Western blotting, consider using whole cell lysates from human cell lines known to express CDKN2A. Since the product is KO validated, including a CDKN2A knockout control lysate is recommended to confirm band specificity.
• The predicted molecular weight of p16INK4a is approximately 16 kDa, though multiple isoforms (8–17 kDa) may be detected due to alternative splicing and post-translational modifications. Optimize gel percentage and transfer conditions for low molecular weight proteins.
• For immunofluorescence (IF/ICC), appropriate fixation (e.g., 4% paraformaldehyde) and permeabilization (e.g., 0.1% Triton X-100) are advised. p16INK4a localizes to both the nucleus and cytoplasm, so imaging should cover whole cell.
• In ELISA applications, the antibody can be used as a capture or detection reagent after validation against the target antigen. Ensure proper coating buffer and blocking steps for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined, one-stop sourcing channel for IVD raw materials. This anti-CDKN2A/p16INK4a polyclonal antibody supports oncology and cell cycle research programs, backed by our capability to supply validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary raw materials. Whether you need rare target antibodies or large-scale production-grade lots, CamelBio bridges your concept-to-clinic needs with reliable procurement solutions.