[KO Validated] Anti-CDKN1B/p27KIP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P46527

Core Product Specifications and Parameters

Biological Background: CDKN1B/p27KIP1 Function and Localization

• Cyclin-dependent kinase inhibitor 1B (p27KIP1) is encoded by the CDKN1B gene and also known as p27Kip1.
• Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Related references: PMID:28666995
• Acts as a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes, contributing to G1 arrest and cell cycle regulation.
• Modulates cyclin D-CDK4 complexes, acting as inhibitor or activator depending on phosphorylation state and stoichiometry.
• Localizes to the nucleus, cytoplasm, and endosomes.
• Widely expressed in tissues; highest levels in skeletal muscle, lowest in liver and kidney (PubMed:8033212). Detected at protein level in kidney (PubMed:15509543). Related references: PMID:8033212, PMID:15509543
• Classified as a tumor suppressor and phosphoprotein; undergoes ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 99–198 of human p27KIP1; verify specificity using knockout (KO) validated models.
• For Western blot, expect a band near 22 kDa; consider enriching nuclear extracts to improve detection.
• In IHC-P, nuclear staining is anticipated; always include positive and negative tissue controls.
• Cross-reactivity with mouse and rat is listed; validate species-specific performance in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CDKN1B/p27KIP1 antibody supports cell cycle and oncology research, and CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your project needs.