[KO Validated] Anti-CDKN1A/p21 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P38936

Core Product Specifications and Parameters

Biological Background: CDKN1A/p21 Function and Localization

• CDKN1A/p21, also known as CIP1, WAF1, SDI1, MDA-6, and CAP20, is a key cyclin-dependent kinase inhibitor involved in cell cycle regulation.
• It plays a critical role in controlling cell cycle progression and DNA damage-induced G2 arrest, mediating p53/TP53-dependent and independent (via CREB3L1) inhibition of proliferation. Related references: PMID:9106657
• CDKN1A binds and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical substrates to block cell cycle progression. It also modulates cyclin D-CDK4 complex assembly and kinase activity in a dose-dependent manner.
• The protein inhibits DNA synthesis by competing with POLD3 for PCNA binding, disrupting DNA polymerase delta function. Related references: PMID:11595739
• In keratinocytes, it negatively regulates CDK4/6-driven phosphorylation of RB1, leading to release of E2F1 and transcription of G1/S phase genes.
• Localized in both the nucleus and cytoplasm, CDKN1A is ubiquitously expressed in adult tissues, with notably lower levels in the brain.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, and it features a zinc-finger domain for metal binding.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-164), which contains the CDK and PCNA binding domains, making this antibody a candidate for detecting functional interactions.
• For WB, consider that p21 migrates around 18-21 kDa; loading controls such as GAPDH or β-actin are recommended.
• Validate reactivity in mouse and rat samples, as cross-reactivity is reported; confirm band specificity in the species of interest.
• For IHC-P, optimize antigen retrieval and antibody dilution; both nuclear and cytoplasmic staining may be observed.
• ELISA applications may require titration of the antibody and antigen to achieve an optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. For cell cycle and DNA damage research, we offer supply solutions for key targets like CDKN1A/p21, helping streamline your assay development from concept to clinic. Explore our catalog for rare target sourcing and technical consulting services.