[KO Validated] Anti-CDK4 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - P11802

Core Product Specifications and Parameters

Biological Background: CDK4 Function and Localization

• Cyclin-dependent kinase 4 (CDK4) is a serine/threonine-protein kinase and a key regulator of the cell cycle G1/S transition. It forms complexes with D-type cyclins to phosphorylate retinoblastoma (RB) family proteins, relieving E2F transcription factors and promoting cell cycle progression.
• Unlike some other CDKs, CDK4 is relatively specific for the G1 phase, where it hypophosphorylates RB1 early in G1, enabling subsequent hyperphosphorylation by cyclin E-CDK2 complexes.
• CDK4 localizes to the cytoplasm, nucleus, and nuclear membrane, and its nuclear translocation is regulated by the ternary complex cyclin D/CDK4/CDKN1B.
• Alternative splicing of CDK4 generates multiple isoforms, and post-translational modifications include acetylation and phosphorylation, which modulate its activity and stability.
• Beyond RB proteins, CDK4 phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity and linking TGF-β signaling to cell cycle control.
• CDK4 integrates various mitogenic and antimitogenic signals, making it a critical node in proliferation control; its dysregulation is frequently observed in cancer, and it is a known disease variant locus.
• Keywords associated with CDK4 include ATP-binding, nucleotide-binding, transferase, phosphoprotein, and reference proteome, highlighting its enzymatic and regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen for this antibody comprises a synthetic peptide corresponding to amino acids 204–303 of human CDK4, which lies in the C-terminal region. The antibody is thus expected to recognize full-length CDK4 and may also detect C-terminal fragments if present.
• For western blotting, a specific band around 34 kDa is anticipated, consistent with the predicted molecular weight of CDK4. Slight variations in observed molecular weight may occur due to post-translational modifications or isoform expression.
• Knockout validation provides strong evidence of specificity. As a best practice, consider including a CDK4 knockout cell lysate as a negative control to confirm band identity in your experimental system.
• In immunoprecipitation, use native lysis conditions to preserve protein–protein interactions if studying CDK4 complexes. The antibody’s rabbit monoclonal origin often ensures high affinity and batch-to-batch consistency.
• For immunohistochemistry (paraffin) experiments, antigen retrieval may be necessary; optimization of retrieval buffer and protocol is recommended for each tissue type.
• Although the antibody is validated for multiple species (human, mouse, rat, monkey), always verify cross-reactivity in your specific sample due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, serving diagnostic manufacturers and research institutes with a comprehensive portfolio. This anti-CDK4 antibody supports investigations into cell cycle regulation and oncology target research, and CamelBio can additionally supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare-target raw materials to accelerate your assay development pipeline.