KO Validated Anti-CDK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• Cyclin-dependent kinase 2 (CDK2), also known as p33 protein kinase or CDKN2, is a serine/threonine-protein kinase essential for cell cycle progression. It drives the G1-S transition and S phase progression by phosphorylating key substrates such as RB1, NPM1, and NPAT. Related references: PMID:10499802, PMID:10995386, PMID:11051553
• CDK2 is activated by cyclin E during early S phase and by cyclin A2 during late S phase/G2, regulating DNA replication, centrosome duplication, and histone gene transcription. Related references: PMID:10995387, PMID:11051553
• Its subcellular localization includes the nucleus, centrosome, Cajal body, cytoplasm, and endosome, reflecting its diverse roles in cell cycle control and DNA metabolism.
• CDK2 participates in DNA damage response and repair by phosphorylating BRCA2, NBN, and ERCC6, thereby modulating homologous recombination and telomere maintenance. Related references: PMID:15800615, PMID:20195506, PMID:21319273, PMID:28216226, PMID:29203878
• CDK2 phosphorylates the epigenetic regulator EZH2, promoting H3K27me3 maintenance and gene silencing, and also regulates the stability and activity of transcription factors like MYC and FOXP3. Related references: PMID:20935635, PMID:19966300
• Post-translational modifications of CDK2 include acetylation and S-nitrosylation, and its kinase activity requires ATP and magnesium. The calculated molecular weight of the human isoform is approximately 33.9 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 200–298) of human CDK2; consider this epitope location when designing blocking or competition experiments.
• For Western blotting (WB), a specific band around 34 kDa is expected; validate detection in a CDK2-expressing cell line (e.g., HeLa, HEK293) and confirm specificity using CDK2 knockout (KO) cell lysates if available.
• In immunohistochemistry-paraffin (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) may need optimization for your tissue type.
• ELISA applications may require titration of both capture and detection antibodies; consider using this polyclonal as part of a matched pair with a monoclonal anti-CDK2.
• Since this is a polyclonal antibody, verify lot-to-lot consistency for critical experiments, especially when transitioning between production batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates IVD development by providing reliable access to essential raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For CDK2-related cell cycle and cancer research, we can source alternative clones, recombinant proteins, and rare target materials to streamline your assay development pipeline. Partner with CamelBio to simplify your procurement process and bring your diagnostics from concept to clinic more efficiently.