[KO Validated] Anti-CD47 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q61735

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• CD47 is a leukocyte surface antigen encoded by the Cd47 gene, also known as integrin-associated protein (IAP). It functions as an adhesive protein mediating cell-cell interactions and as a receptor for thrombospondin-1 (THBS1), modulating integrin signaling through heterotrimeric G proteins.
• CD47 is involved in diverse biological processes including signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation.
  Related references: PMID:20610415, PMID:23591719, PMID:27742621
• It modulates pulmonary endothelin EDN1 signaling, influencing cardiovascular function.
  Related references: PMID:27742621
• In response to THBS1, CD47 inhibits nitric oxide (NO) signaling, thereby supporting blood pressure as a pressor agent.
  Related references: PMID:20610415
• CD47 positively modulates FAS-dependent apoptosis in T-cells, likely by enhancing FAS clustering.
  Related references: PMID:15917238
• It suppresses angiogenesis and may contribute to metabolic dysregulation during normal aging.
  Related references: PMID:32679764
• CD47 prevents premature elimination of red blood cells, playing a role in immune evasion.
  Related references: PMID:10856220
• Located on the cell membrane, CD47 is a glycosylated and phosphorylated transmembrane protein with immunoglobulin domains; it undergoes alternative splicing and plays roles in synaptic plasticity and cell adhesion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19–140 of mouse CD47, which encompasses the extracellular domain. This design supports recognition of the native surface protein in applications such as IHC-P and ELISA.
• For western blot (WB), expect a band at approximately 33–35 kDa; consider that glycosylation may cause slight shifts. Use appropriate positive and negative controls to confirm specificity, especially when analyzing tissues with varying CD47 expression.
• For immunohistochemistry (IHC-P), perform antigen retrieval as per standard protocols and validate staining patterns in known positive tissues (e.g., spleen, thymus).
• In ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and detection systems for your specific assay format.
• As this is a KO-validated clone, it provides a reliable baseline for specificity, but always reconfirm performance in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw material sourcing, from concept to clinic. For immune checkpoint targets like CD47, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need established catalog products or support for rare target sourcing, CamelBio streamlines procurement and supports your diagnostic development pipeline.