KO Validated Anti-CD147/BSG Polyclonal Antibody for WB, ELISA - P35613

Core Product Specifications and Parameters

Biological Background: CD147/BSG (Basigin) Function and Localization

• Basigin (CD147/BSG) is a transmembrane glycoprotein and member of the immunoglobulin superfamily, also known as EMMPRIN, 5F7, TCSF, and HAb18G. It functions as a receptor and cell adhesion molecule.
• Acts as a retinal cell surface receptor for NXNL1, essential for normal retinal development and cone photoreceptor survival by enhancing aerobic glycolysis in photoreceptors (PubMed:25957687).
  Related references:
  PMID:25957687
• Functions as a potent stimulator of IL6 secretion in multiple cell lines including monocytes (PubMed:21620857).
  Related references:
  PMID:21620857
• Serves as the erythrocyte receptor for Plasmodium falciparum RH5, essential for merozoite invasion; binding triggers BSG dimerization and an intracellular Ca2+ increase (PubMed:22080952, 26195724, 28409866).
  Related references:
  PMID:22080952 PMID:26195724 PMID:28409866
• Targets monocarboxylate transporters SLC16A1/GLUT1, SLC16A11, and SLC16A12 to the plasma membrane, facilitating lactate transport and metabolic regulation (PubMed:17127621, 21778275, 28666119).
  Related references:
  PMID:17127621 PMID:21778275 PMID:28666119
• Signaling receptor for cyclophilins PPIA/CYPA and PPIB/CYPB, mediating chemotaxis and adhesion of immune cells (PubMed:11688976, 11943775).
  Related references:
  PMID:11688976 PMID:11943775
• Plays a key role in tumor progression by stimulating extracellular matrix metalloproteinase production and enhancing VEGF signaling through coreceptor activity with KDR/VEGFR2 (PubMed:11992541, 12553375, 15833850, 25825981, 19837976).
  Related references:
  PMID:11992541 PMID:12553375 PMID:15833850 PMID:25825981 PMID:19837976
• Facilitates entry of measles virus, human cytomegalovirus, and SARS-CoV-1 via interaction with cyclophilin A; it is not required for SARS-CoV-2 infection. Also involved in HIV-1 infection.
• Subcellular localization includes cell membrane (basolateral), melanosome, photoreceptor inner/outer segments, endosome, and endoplasmic reticulum membrane. Highly expressed in erythrocytes, melanoma cell lines, heart, kidney, skeletal muscle, testis, bone marrow, fetal liver, lung, and thymus; retina-specific expression in cone photoreceptors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 45-200), enabling recognition of both native and denatured CD147. For WB, expect a band around 42 kDa, although glycosylation may cause slight shifts. KO validation confirms specificity; consider using appropriate positive and negative controls.
• For ELISA applications, the antibody may be used as either capture or detection reagent; evaluate optimal coating concentrations and pairing with matched detection antibodies in your assay system.
• Validate performance in biologically relevant sample types such as melanoma cell lines, erythrocyte lysates, or tissues with high CD147 expression (e.g., heart, kidney).
• Avoid repeated freeze-thaw cycles; store as recommended upon receipt (shipped on ice bag).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive sourcing solution for IVD raw materials, covering every stage from concept to clinic. Beyond this CD147/BSG polyclonal antibody, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Let CamelBio accelerate your immunoassay development with our B2B expertise and reliable supply chain.