[KO Validated] Anti-CD133 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O43490

Core Product Specifications and Parameters

Biological Background: Prominin-1 Function and Localization

• Prominin-1 (also known as AC133, CD133) is a transmembrane glycoprotein encoded by the PROM1 gene; alternative splicing generates multiple isoforms with distinct tissue distributions.
• It is involved in cell differentiation, proliferation, and apoptosis. Related references: PMID:24556617
• The protein binds cholesterol in plasma membrane microdomains and contributes to the organization of the apical plasma membrane in epithelial cells.
• During early retinal development, prominin-1 acts as a key regulator of disk morphogenesis, underscoring its importance in photoreceptor structure.
• In neuroblastoma cells, it suppresses neuronal differentiation (neurite outgrowth) in a RET-dependent manner. Related references: PMID:20818439
• Subcellular localization includes the apical cell membrane, microvilli, cilia, photoreceptor outer segments, and the endoplasmic reticulum–Golgi intermediate compartment.
• Isoform 1 is a well-established marker for CD34+ hematopoietic stem and progenitor cells, whereas isoform 2 shows predominant expression in fetal liver, skeletal muscle, kidney, heart, and adult pancreas, kidney, liver, lung, and placenta.
• Post-translational modifications such as acetylation, phosphorylation, and glycosylation have been identified, which may influence its function and molecular weight in assays.
• Mutations in PROM1 are associated with inherited retinal dystrophies, including cone-rod dystrophy, retinitis pigmentosa, and Stargardt disease.

Experimental Guidance and Technical Tips

• The immunogen (aa 179–433) targets the extracellular domain, likely beneficial for surface labeling in IF/ICC; ensure optimal fixation and permeabilization conditions for cellular localization studies.
• For western blot, note that CD133 is heavily glycosylated; the theoretical mass is ~97 kDa, but the observed band may migrate higher. Use reducing/denaturing conditions and validate with a positive control (e.g., stem cells) alongside the provided KO‑validated negative control strategy.
• When performing immunoprecipitation, mild lysis buffers that preserve membrane protein integrity are recommended, and antibody concentration should be optimized for each experimental system.
• For ELISA, the antibody can be evaluated as a capture or detection reagent; consider pairing with a matched detection antibody and validating with recombinant CD133 protein.
• As a rabbit polyclonal, lot‑to‑lot variability is possible; it is good practice to titrate each new lot for the intended application and confirm specificity in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, supporting every stage from concept to clinic. Complement this CD133 antibody with our portfolio of validated antibody pairs, optimized monoclonals/polyclonals, and bulk ancillary reagents tailored for stem cell and cancer research. Contact us for rare target sourcing or technical consulting to expedite your diagnostic development.