KO Validated Anti-Caspase-9 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P55211

Core Product Specifications and Parameters

Biological Background: Caspase-9 Function and Localization

• Caspase-9 is a member of the cysteine-aspartic acid protease (caspase) family that functions as a key initiator caspase in the intrinsic apoptotic pathway. It is encoded by the CASP9 gene (GeneID: 842) and is also known as Apoptotic protease Mch-6, APAF-3, and ICE-LAP6.
• Upon binding to Apaf-1, caspase-9 forms the apoptosome and undergoes activation. It then cleaves and activates downstream executioner caspases, primarily caspase-3 and caspase-7, to execute apoptosis.
• Caspase-9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner. It also proteolytically cleaves poly(ADP-ribose) polymerase (PARP) and BIRC6. Cleavage of BIRC6 occurs following inhibition of BIRC6-caspase binding by DIABLO/SMAC. Related references: PMID:36758105 PMID:36758106
• An alternatively spliced isoform (isoform 2) lacks catalytic activity and acts as a dominant-negative inhibitor of caspase-9, providing a regulatory mechanism.
• Tissue expression is ubiquitous, with highest levels in the heart (specifically in myocytes), moderate expression in liver, skeletal muscle, and pancreas, and low levels in other tissues.
• As a zymogen, caspase-9 requires proteolytic cleavage for activation. It is further regulated by phosphorylation and ubiquitylation. The protein is classified as a thiol protease and its 3D structure has been resolved.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody covers amino acids 139–416, which includes the catalytic domain. Therefore, the antibody may detect both the full-length proform (~46 kDa) and cleaved active fragments. Consider including appropriate controls when assessing caspase activation.
• For Western blotting, a primary band near 46 kDa is expected in untreated samples; additional smaller bands may appear upon induction of apoptosis. Validate the specific pattern in your model system.
• This antibody has been KO validated, indicating reliable specificity for Caspase-9 in human, mouse, and rat samples. It is suitable for detecting endogenous levels.
• In IF/ICC experiments, caspase-9 localizes predominantly to the cytoplasm but may be observed in the nucleus under certain apoptotic conditions. Optimize fixation and permeabilization protocols accordingly.
• For ELISA applications, evaluate this antibody as part of a matched pair with other anti-Caspase-9 clones to achieve optimal sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For apoptosis research, we supply a comprehensive range of antibodies including this validated anti-Caspase-9 clone, along with recombinant proteins, matched antibody pairs, and bulk ancillary reagents. Our sourcing capabilities extend to rare and custom targets, ensuring you can secure all necessary components efficiently.