KO Validated Anti-Carbonic Anhydrase 2 (CA2) Rabbit Polyclonal Antibody for WB, ELISA - P00918

Core Product Specifications and Parameters

Biological Background: Carbonic Anhydrase 2 (CA2) Function and Localization

• Carbonic anhydrase 2 (CA2) is a cytosolic enzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, a reaction critical for pH regulation, ion transport, and bone resorption. Related references: PMID:11327835, PMID:11802772, PMID:11831900

• CA2 can also hydrate cyanamide to urea, indicating a broader substrate specificity. Related references: PMID:10550681, PMID:11015219

• The protein stimulates the chloride-bicarbonate exchange activity of SLC26A6, linking it to epithelial ion transport. Related references: PMID:15990874

• CA2 is essential for osteoclast differentiation and bone resorption, with mutations causing osteopetrosis. Related references: PMID:15300855

• Subcellular localization includes cytoplasm and cell membrane, as indicated by UniProt keywords and experimental data.

• CA2 is a zinc metalloenzyme and is subject to post-translational modifications including acetylation and phosphorylation.

• It is involved in fluid secretion into the anterior chamber of the eye and contributes to intracellular pH regulation in the duodenal epithelium during proton-coupled peptide absorption.

• The protein belongs to the alpha-carbonic anhydrase family and is also known as carbonic anhydrase II (CA-II), carbonate dehydratase II, and CAC.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-250 of human CA2, which may confer reactivity across species with high sequence homology, such as mouse.
• For Western blot, a band around 29 kDa is anticipated; consider using appropriate positive controls and validate in the relevant sample system.
• In ELISA applications, optimization of coating concentration and detection conditions is recommended, as performance may vary depending on the assay format.
• To ensure specificity, it is advisable to perform knockdown or knockout validation in the cellular system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive platform for sourcing IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance with rare target raw-material procurement. This anti-CA2 polyclonal antibody supports research into carbonic anhydrase function and disorders such as osteopetrosis; our team can help connect you with additional reagents and technical services to streamline your diagnostic development workflow.