[KO Validated] Anti-Calnexin Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P27824

Core Product Specifications and Parameters

Biological Background: Calnexin Function and Localization

• Calnexin (also known as IP90, p90, p88) is a calcium-binding, lectin-like chaperone of the endoplasmic reticulum (ER) that specifically interacts with newly synthesized monoglucosylated glycoproteins.
• It acts as a central quality-control component by retaining incorrectly folded or unassembled protein subunits in the ER, thereby facilitating proper protein folding and assembly.
• Calnexin is implicated in T-cell biology: it associates with incomplete T-cell antigen receptor complexes in immature thymocytes and is thought to contribute to thymocyte maturation signaling.
• Beyond the ER, Calnexin has been detected at the mitochondrion and melanosome membranes, and may participate in receptor-mediated endocytosis at synapses.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, palmitoylation, disulfide bond formation, and ubiquitination, which may regulate its chaperone activity and localization.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 68 kDa. Include appropriate positive and negative (KO) control lysates to verify specificity.
• The antibody is suitable for immunofluorescence/immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P) on human samples; optimize fixation and permeabilization protocols for your specific cell or tissue type.
• As the immunogen resides within the N-terminal region (aa 1-100), the antibody may detect N-terminal cleavage products or isoforms; consider running full-length controls.
• Since cross-reactivity is limited to human, carefully validate reactivity if applying to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing solution for high-quality IVD raw materials. This anti-Calnexin monoclonal antibody is part of a broad portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects requiring rare or custom targets in protein folding and quality control pathways, CamelBio provides one-on-one technical consulting and global sourcing support.