KO Validated Anti-BRG1/SMARCA4 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P51532

Core Product Specifications and Parameters

Biological Background: BRG1/SMARCA4 Function and Localization

• BRG1 (also known as SMARCA4, BAF190A, SNF2-beta) is a catalytic ATPase subunit of the SWI/SNF chromatin remodeling complex, which alters DNA-nucleosome contacts to regulate transcription of select genes (PubMed:15075294, PubMed:29374058, PubMed:30339381). Related references: PMID:15075294, PMID:29374058, PMID:30339381
• It is a key component of the CREST-BRG1 complex that orchestrates calcium-dependent transcriptional control, balancing repressor and activator complexes at promoters like c-FOS and NR2B (By similarity).
• SMARCA4 belongs to the neural progenitors-specific (npBAF) and neuron-specific (nBAF) chromatin remodeling complexes, playing critical roles in neural stem cell proliferation and neuronal differentiation.
• The protein contains a bromodomain, ATP-binding domain, and other functional motifs; it undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, as evidenced by its presence in the phosphoproteome and ubiquitin conjugates.
• It localizes to the nucleus and has been shown to interact with RNA in a promiscuous manner, suggesting additional regulatory roles (By similarity).
• SMARCA4 acts as a corepressor of ZEB1 to promote epithelial-mesenchymal transition (EMT), and its expression is detected in E-cadherin-negative cells in colorectal carcinomas (PubMed:20418909). Related references: PMID:20418909
• Mutations in SMARCA4 are associated with intellectual disability and deafness, underscoring its importance in development.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated against a recombinant protein spanning amino acids 680–770 of human BRG1/SMARCA4. Users may find it effective in applications such as WB, IHC-P, IF/ICC, IP, and ELISA.
• For WB detection of the 185 kDa protein, it is advisable to use a low-percentage (e.g., 6–8%) SDS-PAGE gel and consider a wet transfer method with extended time.
• For immunohistochemistry and immunofluorescence, appropriate antigen retrieval and fixation protocols should be determined empirically.
• As a KO-validated reagent, it may reduce off-target binding; however, specificity should be confirmed in the user's own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For chromatin remodeling and transcription research, we offer a range of SMARCA4-related products to support your assay development needs. Whether you need rare target reagents or scalable supply, CamelBio bridges the gap from concept to clinic.