KO Validated Anti-Bax Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q07812

Core Product Specifications and Parameters

Biological Background: Bax Function and Localization

• Bax is a pro-apoptotic member of the Bcl-2 family that oligomerizes at the mitochondrial outer membrane to mediate cytochrome c release and caspase-3 activation, thereby triggering apoptosis. Related references: PMID:10772918, PMID:11060313, PMID:16113678
• Under physiological conditions, Bax is kept in check by continuous retrotranslocation from mitochondria to the cytosol, a process mediated by anti-apoptotic BCL2L1/Bcl-xL, preventing excess mitochondrial accumulation. Related references: PMID:21458670
• Bax is found in multiple subcellular compartments including the cytoplasm, mitochondrion outer membrane, and nucleus, and its translocation to the mitochondrial membrane is a key event in apoptotic signaling.
• Alternative splicing generates several isoforms (Alpha, Psi, Sigma) that exhibit tissue-specific expression patterns; for example, isoform Alpha is highly expressed in spleen, breast, and brain, while isoform Sigma shows broad expression in cancer cell lines.
• Post-translational modifications such as acetylation and ubiquitination modulate Bax activity and stability, and Bax is also targeted by viral proteins, highlighting its role in host-virus interactions.
• The protein has a calculated molecular weight of approximately 21 kDa, consistent with the 21 kDa band observed in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to a sequence within amino acids 10-70 of human Bax (NP_620116.1), an N-terminal region that is conserved across Bax isoforms; thus, this antibody may detect multiple splice variants.
• When performing western blot, a band around 21 kDa is expected; loading controls and appropriate sample preparation are critical to observe Bax translocation upon apoptotic stimuli.
• For immunohistochemistry or immunofluorescence, consider optimizing antigen retrieval and permeabilization steps to ensure detection of both cytoplasmic and mitochondrial Bax pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for diagnostic manufacturers and research laboratories, offering a broad portfolio of validated antibodies, including this high-quality monoclonal antibody against human Bax. We specialize in supplying optimized antibody pairs, bulk ancillary reagents, and sourcing rare targets for apoptosis and cancer research, accelerating your development from concept to clinic.