KO Validated Anti-Bad Rabbit Polyclonal Antibody for WB and ELISA - Q92934

Core Product Specifications and Parameters

Biological Background: Bad Function and Localization

• Bad (Bcl2-associated agonist of cell death) is a pro-apoptotic member of the Bcl-2 protein family.
• It promotes cell death by competitively binding to anti-apoptotic proteins Bcl-X(L), Bcl-2, and Bcl-W, thereby freeing pro-apoptotic BAX to induce mitochondrial outer membrane permeabilization.
• Alternative names include BBC2, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, and Bcl2 antagonist of cell death.
• The protein localizes to the mitochondrial outer membrane and cytoplasm, with its activity regulated by subcellular translocation and post-translational modifications.
• Phosphorylation, acetylation, and methylation modulate Bad's interactions and apoptotic function, linking growth factor receptor signaling to the cell death machinery.
• Widely expressed across human tissues, underscoring its fundamental role in cellular homeostasis.
• The human BAD gene (GeneID: 572) encodes a protein with a molecular weight of approximately 18 kDa (18392 Da, UniProt Q92934).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 68–168) includes the BH3 domain critical for Bad's pro-apoptotic function; the antibody may recognize this functionally relevant region.
• For Western blot, use whole cell lysates or mitochondrial fractions under reducing conditions; expect a band near 18 kDa. Consider using known apoptosis inducers as positive controls.
• For ELISA, validate assay conditions with recombinant Bad protein or the immunizing peptide to confirm reactivity.
• The antibody is knock-out validated, supporting target specificity; however, as a polyclonal, lot-to-lot consistency should be internally verified for quantitative experiments.
• Mouse cross-reactivity is reported; validate performance in mouse cell lines or tissues of interest before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Bad antibody supports apoptosis and cell death research, and we can supply it together with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us for tailored procurement solutions that streamline your development workflow.