[KO Validated] Anti-ARHGEF1 Rabbit Polyclonal Antibody - Q92888

Core Product Specifications and Parameters

Biological Background: ARHGEF1 Function and Localization

• ARHGEF1, also known as p115-RhoGEF, Sub1.5, or 115 kDa guanine nucleotide exchange factor, is a multidomain protein encoded by the ARHGEF1 gene.
• It acts as a bi-functional regulator of RhoA GTPase: functioning as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as a guanine nucleotide exchange factor (GEF) for RhoA (PubMed:30521495, PubMed:8810315, PubMed:9641915).
  Related references:
  PMID:30521495
  PMID:8810315
  PMID:9641915
• ARHGEF1 mediates angiotensin-2-induced RhoA activation (PubMed:20098430).
  Related references:
  PMID:20098430
• In germinal center B cells, it participates in S1PR2-dependent signaling that inhibits growth and migration.
• Subcellularly, ARHGEF1 localizes to the cytoplasm and membrane.
• It is ubiquitously expressed across human tissues.
• The protein contains a coiled coil domain, undergoes alternative splicing, and is regulated by phosphorylation (keywords: Alternative splicing, Coiled coil, Phosphoprotein). 3D structures are available, and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 783-912 of human ARHGEF1 (NP_004697.2). This C-terminal region may be conserved in known isoforms and rat orthologs, contributing to the observed cross-reactivity.
• For Western blot, the theoretical molecular weight is ~102 kDa. Use a validated positive control lysate from human or rat cell lines expressing ARHGEF1, and confirm band specificity with KO cell lysate if available.
• This antibody is suitable for IHC-P and ELISA. For IHC, optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer pH 6.0) and include appropriate tissue controls.
• As a polyclonal antibody, performance may vary between lots; consider validating each new lot for your specific assay requirements, especially for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This ARHGEF1 polyclonal antibody supports research into RhoA signaling pathways relevant to cardiovascular disease and oncology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials to streamline your assay development and production needs.