[KO Validated] Anti-AMPKα2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P54646

Core Product Specifications and Parameters

Biological Background: AMPKα2 Function and Localization

• Serves as the catalytic subunit of AMP-activated protein kinase (AMPK), a master energy sensor that activates catabolic pathways and suppresses anabolic pathways when intracellular ATP declines. Related references: PMID:17307971, PMID:17712357
• Controls lipid and cholesterol biosynthesis by directly phosphorylating and inactivating key metabolic enzymes, including ACACA, ACACB, HMGCR, and LIPE. Related references: PMID:7959015, PMID:34077757
• Modulates insulin signaling and systemic glucose homeostasis through phosphorylation of IRS1, PFKFB2/3, and by promoting GLUT4 translocation to the plasma membrane. Plays a role in insulin receptor internalization. Related references: PMID:25687571
• Acts as a transcriptional and epigenetic regulator by phosphorylating multiple transcription co-activators and chromatin modifiers, including CRTC2, FOXO3, HDAC5, p53, SREBF1/2, PPARGC1A, and histone H2B. Related references: PMID:11518699, PMID:11554766, PMID:15866171
• Under nutrient stress, suppresses mTORC1 signaling by phosphorylating TSC2 and RPTOR, and promotes autophagy via ULK1 and WDR45 phosphorylation. Additionally, inactivates non-canonical mTORC1 through FNIP1, enhancing lysosomal gene expression. Related references: PMID:14651849, PMID:20160076, PMID:21205641, PMID:28561066, PMID:37079666
• Regulates circadian rhythm by destabilizing CRY1 and modulates Wnt signaling by stabilizing CTNNB1. Also participates in actin cytoskeleton remodeling and cell migration. Related references: PMID:17486097
• Localizes to the cytoplasm, nucleus, and late endosomes. Belongs to the serine/threonine protein kinase family and undergoes phosphorylation and ubiquitination, reflecting its tightly controlled activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270–343 of human AMPKα2, a conserved region that supports detection across human, mouse, and rat samples. Sequence alignment is recommended when extending to other species.
• For Western blot, a band of approximately 62 kDa is expected; KO validation confirms specificity. Include appropriate positive and negative controls.
• In immunocytochemistry/immunofluorescence, anticipate predominantly cytoplasmic and nuclear staining; validate staining patterns in your cell model.
• For ELISA, as a polyclonal antibody it may serve as a capture or detection reagent; pairwise titration with matched antigen or antibody pairs is suggested.
• Polyclonal antibodies can exhibit lot-to-lot variability; for quantitative assays, consider using a characterized reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, supporting diagnostic kit developers, research institutions, and clinical laboratories. This anti-AMPKα2 antibody is part of our expanding catalog of high-quality immunological reagents for energy metabolism and autophagy research. Beyond antibodies, we supply validated antibody pairs, antigens, and ancillary reagents at bulk scale, and assist with sourcing rare-target raw materials to accelerate your assay development from concept to clinic.