[KO Validated] Anti-AMPKα1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q13131

Core Product Specifications and Parameters

Biological Background: AMPKα1 Function and Localization

• Encoded by the PRKAA1 gene, AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1) is also known as ACACA kinase, HMGCR kinase, and tau-protein kinase PRKAA1.
• Functions as the catalytic subunit of AMPK, a key energy sensor that activates energy-producing pathways and inhibits energy-consuming processes in response to low intracellular ATP.
  Related references:
  PMID:17307971
  PMID:17712357
  PMID:24563466
• Directly phosphorylates and inactivates metabolic enzymes involved in lipid synthesis (e.g., ACACA, ACACB, HMGCR, LIPE) and regulates cholesterol synthesis.
  Related references:
  PMID:34077757
• Modulates transcription and chromatin structure by phosphorylating transcription factors and histones, including histone H2B at Ser-36, to reprogram gene expression.
  Related references:
  PMID:11518699
  PMID:11554766
  PMID:15866171
• Negatively regulates mTORC1 signaling and stimulates autophagy under nutrient limitation via phosphorylation of TSC2, RPTOR, WDR24, and ULK1.
  Related references:
  PMID:14651849
  PMID:18439900
  PMID:20160076
  PMID:21205641
  PMID:36732624
  PMID:37079666
• Localizes to the cytoplasm, nucleus, and late endosomes, consistent with its multifaceted roles in metabolic signaling and trafficking.
• Keywords: AMPKα1 is an ATP-binding, serine/threonine kinase that participates in autophagy, lipid metabolism, chromatin regulation, cell cycle control, and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 381-480, a region near the C-terminus of human AMPKα1. This may influence detection in truncation mutants or isoform-specific recognition; validate in the relevant sample system.
• For Western blotting (WB), the predicted molecular weight is ~64 kDa. Use fresh protease/phosphatase inhibitors and consider loading controls such as β-actin.
• Knockout (KO) validation data support specificity in human samples; KO cell lysates are recommended as negative controls.
• For immunoprecipitation (IP), pre-clearing lysates and using a low-speed centrifugation step can reduce non-specific binding.
• In immunocytochemistry/immunofluorescence (IF/ICC), fixation with 4% PFA and permeabilization with 0.1% Triton X-100 are typical starting conditions.
• ELISA, IHC-P, and IF/ICC applications may require antigen retrieval or protein coating optimization; titrate primary antibody for optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat AMPKα1 is indicated; however, sequence alignment of the immunogen region is advised to confirm consistency across species.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including this anti-AMPKα1 antibody.
• Backed by rigorous validation and batch-to-batch consistency, our monoclonal antibodies, antigen pairs, and ancillary reagents accelerate assay development for energy metabolism and metabolic disease research.
• For custom or bulk requirements, CamelBio’s technical and supply chain expertise streamlines the transition from research biomarker to clinical diagnostic application.